Nucleosomes, histones & chromatin

著者

• Wu, Carl
• Allis, C. David

書誌事項

Nucleosomes, histones & chromatin

edited by Carl Wu, C. David Allis

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 512-513）

Academic Press, 2012

• Pt. A
• Pt. B

内容説明・目次

巻冊次

Pt. B ISBN 9780123919380

内容説明

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers nucleosomes, histones and chromatin and has chapters on dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA, digital DNase technology, and genome-wide analysis of chromatin transition.

目次

• 18. TBC 19. Quantitative affinity measurements for chromatin binding modules and their cognate marks-- pull-downs/ITC/FP/SPR 20. TBC 21. Modified Lysine Analogs for the study of chromatin modifying enzymes and nucleosome structure 22. Map the exact polydenylation site of mRNA transcripts 23. Analysis of the mechanism of chromatin remodeling by 24. TBC 25. Dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA 26. Mechanisms of ATP-dependent chromatin remodelling revealed by high-resolution optical tweezers 27. Enzymatic analysis of the Tet proteins by TLC and mass spectrometry 28. Investigating histone modifications using quantitative mass spectrometry 29. Single-molecule studies of chromatin remodeling 30. Genome-wide mapping of nucleosomes, chromatin modifications and chromatin-modifying enzymes 31. Digital DNase technology 32. Genome-wide Analysis of Chromatin Transitions
• Integration of Static and Dynamic Approaches 33. Mapping the transcription machinery on the yeast genome 34. TBC

巻冊次

Pt. A ISBN 9780123919403

内容説明

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

目次

1. Methods applied to the study of Protein Arginine Methylation 2. TBC 3. Genome-wide mapping of nucleosomes in budding yeast 4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology 5. TBC 6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins 7. Peptide microarrays to interrogate the aEURO~histone codeaEURO (TM) 8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS 9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry 10. Measuring nucleosome turnover dynamics genome-wide 11. Comparative mRNA expression-profiling of chromatin factors 12. High-Throughput MAPit Single-Molecule Footprinting 13. Genome-wide in vitro reconstitution 14. Quantitating interactions of nuclear factors with chromatin 15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex 16. TBC 17. TBC

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詳細情報

• NII書誌ID(NCID): BB10382684
• ISBN:
  • 9780123919403
  • 9780123919380
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam ; Tokyo
• ページ数/冊数: 2 v.
• 大きさ: 23 cm
• 分類:
  • DC23 : 572.8633
• 件名:
  • LCSH : DNA -- Structure-activity relationships
• 親書誌ID:
  • BA00197543