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Pt. A ISBN 9780123942906
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, Terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.
目次
Steady-state kinetic characterization of sesquiterpene synthases by gas chromatography-mass spectroscopy
Automating gene library synthesis by structure-based combinatorial protein engineering (SCOPE): examples from plant sesquiterpene synthases
In planta Transient Expression Analysis of Monoterpene Synthases
Natural Rubber Biosynthesis in Plants: Rubber Transferase
Discovery and Characterization of Terpenoid Biosynthetic Pathways of Fungi
Menaquinone biosyntheses in microorganisms
Diversity and analysis of bacterial terpene synthases
Platensimycin and platencin biosynthesis in Streptomyces platensis, showcasing discovery and characterization of novel bacterial diterpene synthases
Strategies for Engineering Plant Natural Products:The Iridoid-Derived Monoterpene Indole Alkaloids of Catharanthus roseus
Discovery and functional analysis of monoterpenoid indole alkaloid pathways in plants
Biochemical Genomics for Gene Discovery in Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy and Related Species
Analysis and modification of ergot alkaloid profiles in fungi
Engineering of glucosinolate biosynthesis: candidate gene identification and validation
Structure, function and evolutionary analyses of the plant type III polyketide synthase family
Engineering of plant type III polyketide synthases
Type III polyketide synthases in microorganisms
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Pt. B ISBN 9780123942913
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.
目次
In vivo production of thiopeptide variants
Microviridin biosynthesis
Cyclotide isolation and characterization
Ribosomally-encoded cyclic peptide toxins from mushrooms
The Pictet-Spengler mechanism involved in the biosynthesis of tetrahydroisoquinoline antitumor antibiotics: novel function for a non-ribosomal peptide synthetase
Discovery and biosynthesis of phosphonate and phosphinate natural products
RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins
Fe(II)-dependent, uridine-5'-monophosphate a-ketoglutarate dioxygenases in the synthesis of 5'-modified nucleosides
Heme-dependent Tailoring Enzymes in Natural Product Biosynthesis: Spectroscopic Characterisation of the L-tryptophan-nitrating Cytochrome P450 TxtE
Oxidative Tailoring Reactions Catalysed by Non-heme Iron-dependent Enzymes: Streptorubin B Biosynthesis as an Example
The rare fluorinated natural products and biotechnological prospects for fluorine enzymology
Enzymatic chlorination and bromination
Prenyltransferases of the dimethylallyltryptophan synthase superfamily
Serine carboxypeptidase-like acyltransferases from plants
4-Methylideneimidazole-5-one-containing aminomutases in enediyne biosynthesis
Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis
High-throughput colorimetric assays for nucleotide sugar formation and glycosyl transfer
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Pt. C ISBN 9780124046344
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The third of 3 volumes covering Natural product biosynthesis by microorganisms and plants.
目次
Methods for the study of endophytic microorganisms from Chinese traditional medicine (TCM) plants
Cyanobacteria as a Source of Natural Products
Isolating antifungals from fungus-growing ant symbionts using a genome-guided chemistry approach
Gamma-butyrolactone and furan signalling systems in Streptomyces
Small molecules that mediate interspecies interactions
Finding and Analysing Plant Secondary Metabolic Gene Clusters
Genomic approaches for interrogating the biochemistry of medicinal plant species
Phylogenetic approaches to natural product structure prediction
Using a virus-derived system to manipulate plant natural product biosynthetic pathways
DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters
Reassembly of functionally intact environmental DNA-derived biosynthetic gene clusters
A toolkit for heterologous expression of metabolic pathways in Aspergillus oryzae
De novo synthesis of high-value sesquiterpenoids in yeast
Streptomyces coelicolor as an expression host for heterologous gene clusters
Towards awakening cryptic secondary metabolite gene clusters in filamentous fungi
Regulatory cross-talk and microbial induction of fungal secondary metabolite clusters
Waking up Streptomyces secondary metabolism by genetic disruption of repressors or constitutive expression of activators
Use and discovery of chemical elicitors that stimulate biosynthetic gene clusters in Streptomyces bacteria
Persister eradication: lessons from the world of natural products
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