Recombinant and in vitro RNA synthesis : methods and protocols
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Bibliographic Information
Recombinant and in vitro RNA synthesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 941）（Springer protocols）
Humana Press , Springer, c2012
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Description and Table of Contents
Description
The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.
Table of Contents
1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold
Luc Ponchon and Frederic Dardel
2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
Luc Ponchon, Genevieve Beauvais, Sylvie Nonin-Lecomte and Frederic Dardel
3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams
4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE
Jo L. Linpinsel and Graeme L. Conn
5. Preparation of Short RNA by in vitro Transcription
Cheng Lu and Pingwei Li
6. Native RNA Purification by Gel Filtration Chromatography
Evan P. Booy, Hui Meng and Sean A. McKenna
7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends
Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka
9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong
10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA
Genevieve Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault
11. Affinity Purification of RNA using an ARiBo Tag
Genevieve Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompre-Brodeur, Vanessa Delfosse and Pascale Legault
12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments
Virginia K. Vachon and Graeme L. Conn
13. In Vitro Transcription of Modified RNAs
Stephanie L. Moon and Jeffrey Wilusz
14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis
N. Ruth Zearfoss and Sean P. Ryder
15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
Ya-Ming Hou
16. Se-derivatized RNAs for X-ray Crystallography
Lina Lin and Zhen Huang
17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs
Luigi Martino and Maria R Conte
18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
Pierre Dagenais and Pascale Legault
19. Splint Ligation of RNA with T4 DNA Ligase
Christopher J. Kershaw and Raymond T. O'Keefe
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