Single-cell analysis : methods and protocols
Author(s)
Bibliographic Information
Single-cell analysis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 853）（Springer protocols）
Humana Press , Springer, c2012
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.
Table of Contents
1. Introduction: Why Analyze Single Cells?
Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett
Part I: Conventional Methods
2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview
Sara Lindstroem
3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry
Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet
4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts
Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom
Part II: Miniaturized Solutions
5. Single-Cell Culture in Microwells
Sara Lindstroem and Helene Andersson-Svahn
6. Iso-Dielectric Separation and Analysis of Cells
Michael D. Vahey and Joel Voldman
7. Single Cell Electroporation Using Microfluidic Devices
Severine Le Gac and Albert van den Berg
8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array
Chi Zhang
9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
Sara Henriksson and Mats Nilsson
10. Droplet Microfluidics for Single-Cell Analysis
Eric Brouzes
11. Screening of Antigen-Specific Antibody Secreting Cells
Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi
12. Analysis of Single Eukaryotic Cells Using Raman Tweezers
Elsa Correia Faria and Peter Gardner
13. Single-Cell Microinjection Technologies
Yan Zhang
14. Ultrasonic Manipulation of Single Cells
Martin Wiklund and Bjoern OEnfelt
Part III: Reviews on Chosen Subjects Within the Field
15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices
Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii
16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
Ayca Yalcin, Yvonne J. Yamanaka, and J. Christopher Love
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