Xenotransplantation : methods and protocols
著者
書誌事項
Xenotransplantation : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 885）（Springer protocols）
Humana Press, c2012
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.
目次
1. Xenotransplantation: An Overview of the Field
Veronica Tisato and Emanuele Cozzi
2. Cloning and Expression Analyses of Pig Genes
Mireia Uribe-Herranz and Cristina Costa
3. Basic Analyses of Proteins of Interest for Xenotransplantation
Mireia Uribe-Herranz and Cristina Costa
4. Studies on Carbohydrate Xenoantigens
Dale Christiansen, Effie Mouhtouris, Paul A. Ramsland, and Mauro S. Sandrin
5. Xenoantibodies and Complement Activity Determinations in Pig to Primate Xenotransplantation
Nieves Domenech
6. Studies on Coagulation Incompatibilities for Xenotransplantation
Cristiana Bulato, Claudia Radu, Paolo Simioni
7. Cellular Studies for in Vitro Modeling of Xenogeneic Immune ResponsesRoberta Sommaggio, Magdiel Perez-Cruz, and Cristina Costa
8. Production of Transgenic and Knock Out Pigs by Somatic Cell Nuclear Transfer
Angelica M. Giraldo, Suyapa Ball, Kenneth R. Bondioli
9. Small Animal Models of Xenotransplantation
Hao Wang
10. Heart Xenotransplantation in Primate Models
Johannes Postrach, Andreas Bauer, Michael Schmoeckel, Bruno Reichart, and Paolo Brenner
11. Xenogeneic Lung Transplantation Models
Lars Burdorf, Agnes M. Azimzadeh and Richard N. Pierson III
12. Thymic Transplantation in Pig-to-nonhuman Primates for the Induction of Tolerance Across Xenogeneic Barriers
Kazuhiko Yamada and Joseph Scalea
13. Isolation of Porcine Pancreatic Islets for Xenotransplantation
Karin Ulrichs, Sibylle Eber, Bianca Schneiker, Sabine Gahn, Armin Strauss, Vasiliy Moskalenko, Irina Chodnevskaja
14. Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantation
Xavier Leveque, Veronique Nerriere-Daguin, Isabelle Neveuand Philippe Naveilhan
15. Hepatocyte Xenotransplantation
Katia R. F. Lima-Quaresma, Andre Gustavo Bonavita, Matheus Kafuri Cytrangulo, Marcelo Alves Pinto, and Luiz Anastacio Alves
16. In Vitro Repair Model of Focal Articular Cartilage Defects in Humans
Diaz Prado SM , Fuentes-Boquete IM , Blanco FJ
17. Potential Zoonotic Infection of PERV in Xenotransplantation
Giada Mattiuzzo, Yashuhiro Takeuchi, Linda Scobie
18. Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation
Jorge Guerra Gonzalez
19. Some Ethical, Social and Legal Considerations of Xenotransplantation
Maria Jorqui Azofra and Carlos Maria Romeo Casabona
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