CiNii Books - Liver stem cells : methods and protocols

Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy. These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.

Table of Contents

Contents Part I Several hepatic progenitor cells: 1) Purification and Culture of Fetal Mouse Hepatoblasts that are Precursors of Mature Hepatocytes and Biliary Epithelial Cells Nobuyoshi Shiojiri and Miho Nitou 2) Clinical Uses of Liver Stem Cells Yock Young Dan 3) Identification and Isolation of Adult Liver Stem/Progenitor Cells Minoru Tanaka and Atsushi Miyajima 4) Isolation and Purification Method of Mouse Fetal Hepatoblasts Luc Gailhouste 5) Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka II. Hepatic differentiation form stem cells: 6) Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells Agnieszka Banas 7) Development of Immortalized Hepatocyte-like Cell from hMSC Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng, 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 3) Identification and Isolation of Adult Liver Stem/Progenitor Cells Minoru Tanaka and Atsushi Miyajima 4) Isolation and Purification Method of Mouse Fetal Hepatoblasts Luc Gailhouste 5) Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka II. Hepatic differentiation form stem cells: 6) Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells Agnieszka Banas 7) Development of Immortalized Hepatocyte-like Cell from hMSC Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng, 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 3) Identification and Isolation of Adult Liver Stem/Progenitor Cells Minoru Tanaka and Atsushi Miyajima 4) Isolation and Purification Method of Mouse Fetal Hepatoblasts Luc Gailhouste 5) Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver Norihisa Ichinohe, Junko Kon, Toshihiro Mitaka II. Hepatic differentiation form stem cells: 6) Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells Agnieszka Banas 7) Development of Immortalized Hepatocyte-like Cell from hMSC Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, , Suradej Hongeng, 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 8) Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages Shohei Wakao, Masaaki Kitada, Yasumasa Kuroda, Mari Dezawa 9) Generation and Hepatic Differentiation of Human iPS Cells Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya 10) Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer Kenji Kawabata, Mitsuru Inamura & Hiroyuki Mizuguchi 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko 11) "Tet-On" System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor Goshi Shiota, Yoko Yoshida, Noriko Matsumoto, Yoshiko Hoshikawa 12) SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato and Maria Luz Martinez-Chantar. Part III BD formation from stem cells: 13) Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix Yuji Nishikawa Part IV. Liver stem cells and hepatocarcinogenesis: 14) Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma Junfang Ji and Xin Wei Wang Part V. Application of liver stem cells for cell therapy: 15) Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage Moubarak Mouiseddine, Sabine Francois, Maamar Souidi and Alain Chapel 16) Engineering of Implantable Liver Tissues Y. Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne, N. Kojima, T. Fujii and T. Niino 17) Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis Yoshio Sakai, Shuichi Kaneko

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