Protein electrophoresis : methods and protocols

Author(s)

• Kurien, Biji T
• Scofield, R. Hal

Bibliographic Information

Protein electrophoresis : methods and protocols

edited by Biji T. Kurien and R. Hal Scofield

（Methods in molecular biology / John M. Walker, series editor, v. 869）（Springer protocols）

Humana Press/Springer, c2012

Note

Includes bibliographical references and index

Description and Table of Contents

Description

Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.

Table of Contents

1. How It All Began -- A Personal History of Gel-Electrophoresis Oliver Smithies 2. Introduction to Protein Electrophoresis Pothur R. Srinivas 3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis J.P. Dean Goldring 4. Protein Solubilization: Attend to the Choice of Lysis Buffer Mandy Peach, Noelle Marsh, and Daniel J. MacPhee 5. Native Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann 6. Cationic Electrophoresis Engelbert Buxbaum 7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins Christian Reichel 8. Tricine-SDS-PAGE Syed R. Haider, Helen J. Reid, and Barry L. Sharp 9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen 10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins Marion L. Greaser and Chad M. Warren 11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis Henghang Tsai and Hon-chiu Eastwood Leung 12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann 13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies Gyorgy Csako 14. Microchip Electrophoresis, with respect to "Profiling of A Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy 15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols Mustafa Celebier, Clara Ibanez, Carolina Simo, and Alejandro Cifuentes 16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra 17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa 18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting Edward P. Trieu and Ira N. Targoff 19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing Yaser Dorri 20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids Gyorgy Csako 21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution Biji T. Kurien and R. Hal Scofield 22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori 23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta 24. Two-Dimensional Difference Gel Electrophoresis Jonathan S. Minden 25. Protein Identification on Archived 2-D Gels Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori 26. Diagonal Electrophoresis for the Detection of Protein Disulfides Brian McDonagh 27. Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes Jean Paul Lasserre and Armelle Menard 28. Immunoelectrophoresis: A Method with Many Faces Gyorgy Csako 29. Cell Surface Protein Biotinylation for SDS PAGE Analysis Giuliano Elia 30. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin 31. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome Xianchun Wang and Songping Liang 32. Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test Yan Su, Lijun Gao, Wenbin Qin 33. Extraction of Proteins from Gels - A Brief Review Biji T. Kurien and R. Hal Scofield 34. A New Algorithm for the Simulation of SDS 2D-PAGE Datasets Emilio Marengo and Elisa Robotti 35. Isolation of Proteins from Polyacrylamide Gels Irene Michalk, Stefanie Koristka, Claudia Arndt, and Michael Bachmann 36. Gel Drying Methods Slava Stamova, Irene Michalk, Holger Bartsch, and Michael Bachmann 37. Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags Biji T. Kurien and R. Hal Scofield 38. A Brief Review of Other Notable Electrophoretic Methods James D. Fesmire 39. Protein Stains and Applications Ranjini K. Sundaram, Natarajan Balasubramaniyan, and Pazhani Sundaram 40. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Anja Feldmann, Holger Bartsch, and Michael Bachmann 41. Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat Biji T. Kurien and R. Hal Scofield 42. Silver Staining Techniques of Polyacrylamide Gels Holger Bartsch, Claudia Arndt, Stefanie Koristka, Marc Cartellieri, and Michael Bachmann 43. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis Han-Min Chen 44. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry Jung-Kap Choi 45. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE Na Na and Jin Ouyang 46. Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins Jennie R. Lill and Victor J. Nesatyy 47. Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping Tripti Khare, Yasmin Chishti, and Lydia A. Finney 48. Fluorescent Staining of Gels Engelbert Buxbaum 49. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni 50. Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain Padmaja Mehta-D'souza 51. Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain Biji T. Kurien, Yaser Dorri, and R. Hal Scofield 52. In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin K. Divakar, G. Nandhini Devi, and Pennathur Gautam 53. Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black J.P. Dean Goldring, Robert G.E. Krause, and David Choveaux 54. Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels Mircea Alexandru Mateescu, Carmen Calinescu, Tien Canh Le, Rodolfo Federico, and Bruno Mondovi 55. Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner Biji T. Kurien and Yaser Dorri 56. A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels Biji T. Kurien and R. Hal Scofield 57. Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein (TM), ProteoSilver (TM), SYPRO (R) Ruby, and Coomassie (R) Brilliant Blue Gel Stains Guang Ren, Carl K. Okerberg, and Suresh T. Mathews 58. Common Artifacts and Mistakes Made in Electrophoresis Biji T. Kurien and R. Hal Scofield

by "Nielsen BookData"

• NCID: BB11546357
• ISBN:
  • 9781617798207
• LCCN: 2012936829
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xiv, 648 p.
• Size: 27 cm
• Classification:
  • LCC : QP519.9.E434
  • LCC : QH506
  • NLM : W1
  • NLM : QU 25
  • DC20 : 574.192
• Subject Headings:
  • LCSH : Electrophoresis -- Laboratory manuals
  • MESH : Electrophoresis, Agar Gel -- methods -- Laboratory Manuals
  • MESH : Proteins -- analysis -- Laboratory Manuals
• Parent Bibliography ID:
  • BA10794673
  • BA88873632