Essential guide to reading biomedical papers : recognising and interpreting best practice
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書誌事項
Essential guide to reading biomedical papers : recognising and interpreting best practice
Wiely-Blackwell, 2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.
Allows the reader to develop the necessary skills to properly evaluate research articles
Coverage of over 30 commonly-used techniques in the biomedical sciences
Global approach and application, with contributions from leading experts in diverse fields
目次
List of contributors ix
Foreword xi
Preface xiii
Acknowledgements xvii
Introduction xix
Section A - Basic principles 1
1 Philosophy of science 3
James Ladyman
2 Ingredients of experimental design 9
Nick Colegrave
3 Statistics: a journey that needs a guide 17
Gordon Drummond
Section B - Cell and molecular 27
4 Organ bath pharmacology 29
Emma Robinson
5 Small vessel myography 39
Tomoko Kamishima and John M Quayle
6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49
Scott H Randell
7 Electron microscopy (TEM and SEM) 59
Paul Verkade
8 Fluorescence microscopy 67
Mark Jepson
9 Intracellular 'sharp' microelectrode recording 77
Helena C Parkington and Harold A Coleman
10 Single electrode voltage-clamp (SEVC) 85
Harold A Coleman and Helena C Parkington
11 Patch clamp recording 95
Neil Bannister and Phil Langton
12 Production of antibodies 105
Elek Molnar
13 Immunocytochemistry and immunohistochemistry 117
Elek Molnar
14 Immunoprecipitation (IP) 129
David Bates
15 Immunoblotting (western) 137
Samantha F. Moore, Joshua S. Savage and Ingeborg Hers
16 Applications of green fluorescent protein (GFP) 147
Mark Jepson
17 Fluorescent measurement of ion activity in cells 153
Helen Kennedy
18 Detection of exocytosis - real time 163
Anja Teschemacher
19 Viral vector transgenesis 173
Anja Teschemacher
20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179
Lucy F. Donaldson
21 In situ hybridisation (ISH) 187
Lucy F. Donaldson
22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
Allison Fulford
23 Transcriptome analysis: microarrays 203
Charles Hindmarch
24 Experimental proteomics 215
Thierry Le Bihan
Section C - In vivo / integrative 229
25 Behavioural methodology 231
Emma Robinson
26 Genetically modified mouse models 241
Nina Balthasar
27 Wireless recording of cardiovascular signals 247
Julian FR Paton and Fiona D McBryde
28 Electrical stimulation methods 253
Jon Wakerley
29 Extracellular recording 261
Jon Wakerley
30 Antidromic identification 271
Jon Wakerley
31 Event-triggered averaging, including spike-triggered averaging 279
Richard Apps
32 Axonal transport tracing of CNS pathways 285
John Crabtree
33 Cardiovascular methods: general considerations for human studies 291
Erica A Wehrwein and Michael J Joyner
34 Measuring cardiac output in humans 299
Erica A Wehrwein and Michael J Joyner
35 Measuring peripheral blood flow in humans 311
Erica A Wehrwein and Michael J Joyner
Index 319
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