Mucins : methods and protocols
著者
書誌事項
Mucins : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 842)(Springer protocols)
Humana Press, c2012
大学図書館所蔵 件 / 全2件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
目次
1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins
Karine Rousseau and Dallas M. Swallow
2. Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies
Julia R. Davies, Claes Wickstroem and David J. Thornton
3. Detecting, Visualising and Quantifying Mucins
Ceri A. Harrop, David J. Thornton and Michael A. McGuckin
4. Mass Spectrometric Analysis of Mucin Core Proteins
Mehmet Kesimer and John K. Sheehan
5. O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates
Thomas A. Gerken
6. Analysis of Assembly of Secreted Mucins
Malin E.V. Johansson and Gunnar C. Hansson
7. MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes
Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey
8.Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides
Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunseng Jin, and Niclas G. Karlsson
9. O-Glycomics: Profoliling and Structural Analysis of Mucin-type O-linked Glycans
Isabelle Breloy
10. O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry
Franz-Georg Hanisch
11. Analysing Mucin Degradation
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline Rudd, Elizabeth Matthews, and Anthony P. Corfield
12. Assessment of Mucus Thickness and Production In Situ
Lena Holm and Mia Phillipson
13. Preservation of Mucus in Histrological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH
Malin E.V. Johansson and Gunnar C. Hansson
14. In vitro Measurements of Mucus Secretion by Colon Explants
Jenny K. Gustafsson, Henrik Sjoevall and Gunnar C. Hansson
15. Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function
David R. Hill and Brian Button
David R. Hill and Brian Button
David R. Hill and Brian Button
David R. Hill and Brian Button
16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis
17. Assessment of Intracellular Mucin Content In Vivo
Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
18. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo
Yong H. Sheng, Sumaira Z. Hasain, Chin Wen Png, Michael A. McGuckin and Sara K. Linden
19. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro
Pablo Augueso and Ilene K. Gipson
16. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
Lubna H. Abdullah, Cedric Wolber, Mehment Kesimer, John K. Sheehan, and C. William Davis
17. Assessment of Intracellular Mucin Content In Vivo
Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
18. Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo
Yong H. Sheng, Sumaira Z. Hasain, Chin Wen Png, Michael A. McGuckin and Sara K. Linden
19. Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro
Pablo Augueso and Ilene K. Gipson
「Nielsen BookData」 より