The cytoskeleton : imaging, isolation, and interaction
Author(s)
Bibliographic Information
The cytoskeleton : imaging, isolation, and interaction
(Neuromethods, 79)(Springer protocols)
Humana Press, c2013
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Includes bibliographical references and index
Description and Table of Contents
Description
The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered. The Cytoskeleton: Imaging, Isolation, and Interaction assembles chapters on general aspects of the cytoskeleton, which are helpful for polishing knowledge of the cytoskeleton in a brief and informative way, and these pieces are flanked by a collection of detailed protocols on diverse emerging techniques including in vivo and in vitro imaging of the cytoskeleton at work as well as proteomics methodologies addressing preparative strategies for the isolation of cytoskeletal components of the central nervous system. Written for the popular Neuromethods series, this work contains the kind of meticulous descriptions and implementation advice to guarantee successful results.
Authoritative and cutting-edge, The Cytoskeleton: Imaging, Isolation, and Interaction provides a compilation of papers written by a team of experts which aims to strengthen our efforts at overcoming at least some of the fatal outcomes of the diseased brain cytoskeleton.
Table of Contents
Part I: Imaging
1. Fluorescence Proteins and Time-Lapse Imaging of the Cytoskeleton
Carsten Theiss and Karl Meller
2. Visualizing In Vitro Trafficking
John Murray
Part II: Interaction, Dynamics, and Proteomics
3. Interaction of the Glycocalyx with the Actin Cytoskeleton
Mia M. Thi, Eno E. Ebong, David C. Spray, and Sylvia O. Suadicani
4. Cytoskeleton Dynamics and Binding Factors
Elena E. Grintsevich and Emil Reisler
5. Principles of Proteomic Approaches to the Cytoskeleton
Christina Loosse, Katrin Marcus, and Bodo Schoenebeck
Part III: The Neuronal Cytoskeleton
6. Analyzing the Cytoskeletal Transcriptome: Sex Differences in Rat Hypothalamus
Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas
7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes
Cantas Alev, Georg Zoidl, and Rolf Dermietzel
8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases
Saar Oz and Illana Gozes
9. Neurofilaments: Properties, Functions, and Regulation
Rodolphe Perrot and Joel Eyer
10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics
Micha E. Spira and Hadas Erez
11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton
Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken
Part IV: Astroglia and the Cytoskeleton
12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact
Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel
13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis
Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson
Part V: Oligodendroglia and Myelin
14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System
Christiane Richter-Landsberg
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas
7. Proteomic Approaches for the Study of Electrical Synapses and Associated Protein-Interaction Complexes
Cantas Alev, Georg Zoidl, and Rolf Dermietzel
8. The Cytoskeleton as a Pharmacological Target in Neurodegenerative Diseases
Saar Oz and Illana Gozes
9. Neurofilaments: Properties, Functions, and Regulation
Rodolphe Perrot and Joel Eyer
10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics
Micha E. Spira and Hadas Erez
11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton
Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken
Part IV: Astroglia and the Cytoskeleton
12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact
Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel
13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis
Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson
Part V: Oligodendroglia and Myelin
14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System
Christiane Richter-Landsberg
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
Saar Oz and Illana Gozes
9. Neurofilaments: Properties, Functions, and Regulation
Rodolphe Perrot and Joel Eyer
10. From an Axon into a Growth Cone after Axotomy: A Model for Cytoskeletal Dynamics
Micha E. Spira and Hadas Erez
11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton
Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken
Part IV: Astroglia and the Cytoskeleton
12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact
Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel
13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis
Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson
Part V: Oligodendroglia and Myelin
14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System
Christiane Richter-Landsberg
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
11. Isolation of the Postsynaptic Density: A Specialization of the Subsynaptic Cytoskeleton
Karl-Heinz Smalla, Patricia Klemmer, and Ursula Wyneken
Part IV: Astroglia and the Cytoskeleton
12. Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact
Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel
13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis
Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson
Part V: Oligodendroglia and Myelin
14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System
Christiane Richter-Landsberg
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
Georg Zoid, Stefan Olk, Andrey Tuchinowitz, and Rolf Dermietzel
13. Reactive Astrocytes, Astrocyte Intermediate Filament Proteins, and Their Role in the Disease Pathogenesis
Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson
Part V: Oligodendroglia and Myelin
14. Microtubules and Associated Proteins in Oligodendrocytes, the Myelin Forming Cells of the Central Nervous System
Christiane Richter-Landsberg
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
15. Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner
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