Cytochrome P450 protocols
著者
書誌事項
Cytochrome P450 protocols
(Methods in molecular biology / John M. Walker, series editor, 987)(Springer protocols)
Humana Press, c2013
3rd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates). Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.
目次
1. Bioluminescent Assays for Cytochrome P450 Enzymes
Douglas S. Auld, Henrike Veith, and James J. Cali
2. In Vitro Drug-Drug Interaction Assay
Michael Zientek and Kuresh Youdim
3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening
Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
4. The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites
Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem
5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators
Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
6. Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches
Qian Cheng and F. Peter. Guengerich
7. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis
Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, and Paul R. Ortiz de Montellano
8. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins
Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
9. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group
Wesley E. Straub, Clinton R. Nishida, and Paul R. Ortiz de Montellano
10. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes
A. Luthra, M. Gregory, Y.V. Grinkova, I.G. Denisov , and S.G. Sligar
11. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles
Linlin Zhao, Besnik Bajrami, and James F. Rusling
12. Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells
M. Teresa Donato and M. Jose Gomez-Lechon
13. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme
Kersten S. Rabe and Christof M. Niemeyer
14. High-Throughput Fluorescence Assay of Cytochrome P450 3A4
Qian Cheng and F. Peter. Guengerich
15. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts
Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler
16. DNA Shuffling of Cytochrome P450 Enzymes
James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
17. Measurement of P450 Difference Spectra Using Intact Cells
Wayne A. Johnston and Elizabeth M.J. Gillam
18. DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production
Nedeljka N. Rosic
19. P450 oxidoreductase - Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein
Vishal Agrawal and Walter L. Miller
20. LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450
Federico Sabbadin, Gideon G. Grogan and Neil C. Bruce
21. Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database
Sarah C. Sim and Magnus Ingelman-Sundberg
22. Simultaneous in vivo Phenotyping of CYP Enzymes
Sussan Ghassabian and Michael Murray
23. Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting
Matthew Hancock and Elizabeth A. Shephard
24. Isolation of Mouse Hepatocytes
Mina Edwards, Lyndsey Houseman, Ian R. Phillips, and Elizabeth A. Shephard
25. Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies
Veronique Laurent, Denise Glaise, Tobias Nubel, David Gilot, Anne Corlu, and Pascal Loyer
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