Cilia
著者
書誌事項
Cilia
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 524-525)
Elsevier/Academic Press, 2013
- Pt. A
- Pt. B
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
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Pt. B ISBN 9780123979445
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
目次
Methods for studying ciliary polarity in Xenopus
Analysis of signaling pathways in mammalian spermatozoa
Analysis of tubulin modification in zebrafish cilia
Analysis of microtubule end-binding proteins in cilia
Small molecule screens using Chlamydomonas flagella
Biochemical and Physiological analysis of axonemal dyneins
Analysis of ciliary assembly and function in Planaria
Reconstitution of flagellar sliding
Comparative genomic analysis of ciliary genes
Analysis of ciliogenesis in Tetrahymena
Measuring the regulation of dynein activity during flagellar motility
Centrosome isolation and proteomics
Probing ciliogenesis using micropatterned substrates
3D structural analysis of flagella/cilia by electron cryo-tomography
Visualizing IFT in mammalian cilia
Ciliogenesis in multiciliated epithelial cells in culture
Methods for analysis of PKD-containing urinary exosomes
Analysis of Interactions between IFT Proteins
Electron microscopy of Intraflagellar Transport
Using the SNAP tag to measure ciliary trafficking
- 巻冊次
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Pt. A ISBN 9780123979452
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.
目次
Electron microscopy of IFT in cilia and flagella
Biochemical analysis of ciliary transport
Visualization of IFT in Chlamydomonas flagella
Visualization of kidney cilia-mediated calcium signaling
Methods for analyzing nodal cilia and flow
Methods for building synthetic cilia-like motor-microtubule arrays
Isolation of IFT particle proteins from Chlamydomonas
Radial Spoke isolation and assays
Biomechanical measurements of kinocilium
Methods for analysis of Calcium/calmodulin signaling in flagella
Ubiquitination of Flagellar Proteins
Visualizing IFT in C. elegans cilia
Proteomic analysis of mammalian primary cilia
TSC and TOR signaling in cilia
Bioinformatic approaches to cilia
Methods for testing cilia function in cell migration
Signaling and waveform in Drosophila sperm flagella
Analysis of import into primary cilia
Patch-Clamp Recording from Mouse and Human Spermatozoa
Optical trap measurements of flagellar membrane motility
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