Cilia

著者

• Marshall, Wallace F.

書誌事項

Cilia

edited by Wallace F. Marshall

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 524-525）

Elsevier/Academic Press, 2013

• Pt. A
• Pt. B

注記

Includes bibliographical references and indexes

内容説明・目次

巻冊次

Pt. B ISBN 9780123979445

内容説明

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

目次

Methods for studying ciliary polarity in Xenopus Analysis of signaling pathways in mammalian spermatozoa Analysis of tubulin modification in zebrafish cilia Analysis of microtubule end-binding proteins in cilia Small molecule screens using Chlamydomonas flagella Biochemical and Physiological analysis of axonemal dyneins Analysis of ciliary assembly and function in Planaria Reconstitution of flagellar sliding Comparative genomic analysis of ciliary genes Analysis of ciliogenesis in Tetrahymena Measuring the regulation of dynein activity during flagellar motility Centrosome isolation and proteomics Probing ciliogenesis using micropatterned substrates 3D structural analysis of flagella/cilia by electron cryo-tomography Visualizing IFT in mammalian cilia Ciliogenesis in multiciliated epithelial cells in culture Methods for analysis of PKD-containing urinary exosomes Analysis of Interactions between IFT Proteins Electron microscopy of Intraflagellar Transport Using the SNAP tag to measure ciliary trafficking

巻冊次

Pt. A ISBN 9780123979452

内容説明

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

目次

Electron microscopy of IFT in cilia and flagella Biochemical analysis of ciliary transport Visualization of IFT in Chlamydomonas flagella Visualization of kidney cilia-mediated calcium signaling Methods for analyzing nodal cilia and flow Methods for building synthetic cilia-like motor-microtubule arrays Isolation of IFT particle proteins from Chlamydomonas Radial Spoke isolation and assays Biomechanical measurements of kinocilium Methods for analysis of Calcium/calmodulin signaling in flagella Ubiquitination of Flagellar Proteins Visualizing IFT in C. elegans cilia Proteomic analysis of mammalian primary cilia TSC and TOR signaling in cilia Bioinformatic approaches to cilia Methods for testing cilia function in cell migration Signaling and waveform in Drosophila sperm flagella Analysis of import into primary cilia Patch-Clamp Recording from Mouse and Human Spermatozoa Optical trap measurements of flagellar membrane motility

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詳細情報

• NII書誌ID(NCID): BB12425449
• ISBN:
  • 9780123979452
  • 9780123979445
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam ; Tokyo
• ページ数/冊数: v.
• 大きさ: 24 cm
• 分類:
  • DC23 : 571.67
• 件名:
  • LCSH : Cilia and ciliary motion
• 親書誌ID:
  • BA00197543