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Pt. C ISBN 9780124058811
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.
目次
The Biological Chemistry of Hydrogen Peroxide
Reactive Oxygen Species in the Activation of MAP Kinases
Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells
In vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes
Methods for Studying Oxidative Regulation of Protein Kinase C
P66Shc, Mitochondria and the Generation of Reactive Oxygen Species
Detecting Disulfide Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide Dependent Protein Kinases by H2O2
Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation
Activation of Nrf2 by H2O2: De novo Synthesis versus Nuclear Translocation
H2O2 in the Induction of NF-k B-Dependent Selective Gene Expression
Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRa
Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway
Investigating the Role of Reactive Oxygen Species in Regulating Autophagy
H2O2: A Chemoattractant?
Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells
Effects of H2O2 on Insulin Signaling and the Glucose Transport System in Mammalian Skeletal Muscle
Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle
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Pt. B ISBN 9780124058828
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the cellular steady-state of H2O2, evaluating peroxiredoxin sensitivity towards inactivation by peroxide substrates, and peroxiredoxins as preferential targets in H2O2-induced signaling.
目次
1. The Cellular Steady-State of H2O2: Latency Concepts and Gradients
2. Evaluating Peroxiredoxin Sensitivity Towards Inactivation by Peroxide Substrates
3. Peroxiredoxins as Preferential Targets in H2O2-Induced Signaling
4. Selenium in the Redox Regulation of the Nrf2 and the Wnt Pathway
5. Selenoprotein W as Biomarker for the Efficacy of Selenium Compounds to Act as Source for Selenoprotein Biosynthesis
6. Peroxiredoxins and Sulfiredoxin at the Crossroads of the No and H2o2 Signaling Pathways
7. Glutathione and g-glutamylcysteine in Hydrogen Peroxide Detoxification
8. Peroxiredoxin-6 and NADPH Oxidase Activity
9. Study of the Signaling Function of Sulfiredoxin and Peroxiredoxin III in Isolated Adrenal Gland: Unsuitability of Clonal and Primary Adrenocortical Cells
10. The Use of HyPer to Examine Spatial and Temporal Changes in H2O2- in High Light-Exposed Plants
11. Analysis of Environmental Stress in Plants with the Aid of Marker Genes for H2O2 Responses
12. The Role of Plant Bax Inhibitor-1 in Suppressing H2O2-Induced Cell Death
13. Comparative Analysis of Cyanobacterial and Plant Peroxiredoxins and their Electron Donors: Peroxidase Activity and Susceptibility to Overoxidation
14. Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant cells: A Method with Virtually Unlimited Potential in Plant Biology
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Pt. A ISBN 9780124058835
内容説明
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.
目次
Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light
Fiona A. Summers, Baozhong Zhao, Douglas Ganini, and Ronald P. Mason
Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems
Vivian S. Lin, Bryan C. Dickinson and Christopher J. Chang
Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe
In vivo Imaging of H2O2 Production in Drosophila
Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging
Electrochemical Biosensors for On-Chip Detection of Oxidative Stress
Electrochemical Detection of H2O2 Formation in Isolated Mitochondria
Detection of H2O2 by Fluorescence Correlation Spectroscopy
Real-Time Monitoring of Reactive Oxygen and Nitrogen Species in a multi-well plate using the diagnostic marker products of specific probes
H2O2 Delivery to Cells: Steady-State versus Bolus Addition
Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling
The Determination aAnd Analysis oOf Site-Specific Rates oOf Mitochondrial Reactive Oxygen Species Production
A Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging
Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.
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