Oral biology : molecular techniques and applications
著者
書誌事項
Oral biology : molecular techniques and applications
（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, v.666）
Humana Press, c2010
- : alk. paper
- : ebook
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注記
Includes bibliographical references and index
Also available online
内容説明・目次
内容説明
It is generally recognized that the knowledge and research base that underpins dentistry lies in the biological and physical sciences. In this context, the major advances in these sciences over the past two decades have come through the application of molecular bi- ogy and nanotechnology. These advances are currently impacting on the diagnosis and treatment of a wide range of human diseases and it is essential that dental research, education, and practice keep pace with this rapidly advancing ?eld. As pointed out by Ford et al. (1): The de?nition of disease is also changing. Previously, disease was understood to be the presence of symptoms or of a particular phenotype. With increasing knowledge of the genetic basis of many diseases, this de?nition is changing to become the presence of a genotype conferring a pre-disposition to clinical symptoms or phenotype (Ford et al. (1)). This changing de?nition of disease means that today's undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting. This naturally positions oral biology as an integral part of any dentally related professional's repertoire of knowledge. There are as many topics in oral biology as there are the number of sites and micro- vironments within the oral cavity.
目次
Part I: Saliva Studies
1. Gene Therapy of Salivary Diseases
Bruce J. Baum, Janik Adriaansen, Ana P. Cotrim, Corinne M. Goldsmith, Paola Perez, Senrong Qi, Anne M. Rowzee, and Changyu Zheng
2. Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications
Bradley Stephen Henson and David T. Wong
3. Proteomic Analyses of Saliva: 2-D Gel Electrophoresis, LC-MS/MS, and Western Blotting
Shen Hu, Jiang Jiang, and David T. Wong
4. Transcriptomic Analyses of Saliva
Viswanathan Palanisamy and David T. Wong
Part II: Oral Microbiology
5. The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques
Jose F. Siqueira, Jr. and Isabela N. Rocas
6. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)
Jose F. Siqueira, Jr., Mitsuo Sakamoto, and Alexandre S. Rosado
7. Protocols to Study the Physiology of Oral Biofilms
Jose A. Lemos, Jacqueline Abranches, Hyun Koo, Robert E. Marquis, and Robert A. Burne
8. Adhesion of Yeast and Bacteria to Oral Surfaces
Richard D. Cannon, Karl M. Lyons, Kenneth Chong, and Ann R. Holmes
9. Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction
Stephen M. Hamlet
10. Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy
Abdul Aziz, Dikesh Parmar, Andrew McNaughton, and Geoffrey R. Tompkins
11. Characterization of Anti-Competitor Activities Produced by Oral Bacteria
Fengxia Qi and Jens Kreth
12. Natural Transformation of Oral Streptococci
Fernanda Cristina Petersen and Anne Aamdal Scheie
13. Use of In Vivo Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis
Shannon M. Wallet, Jin Chung, and Martin Handfield
14. Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System
Nicholas C.K. Heng and Jo-Ann L. Stanton
15. Useof a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps
Erwin Lamping and Richard D. Cannon
Part III: Cells and Tissues
16. Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression
Katja Narhi and Irma Thesleff
17. A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, and P. Mark Bartold
18. Preclinical Methods for the Evaluation of Periodontal Regeneration In vivo
Yang-Jo Seol, Gaia Pellegrini, Lea M. Franco, Po-Chun Chang, Chan-Ho Park, and William V. Giannobile
19. Proteomic Analysis of Dental Tissue Microsamples
Jonathan E. Mangum, Jew C. Kon, and Michael J. Hubbard
20. Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry
Pauline J. Ford
21. Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens
Koichi Tabeta and Kazuhisa Yamazaki
22. Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease
Kazuhisa Yamazaki and Harue Ito
23. Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts
Patty Chou and Trudy J. Milne
24. The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases
Moritz Kebschull and Panos N. Papapanou
25. Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software
Ryan T. Demmer, Paul Pavlidis, and Panos N. Papapanou
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