Amyloid proteins : methods and protocols

著者

• Sigurdsson, Einar M
• Calero, Miguel
• Gasset, María

書誌事項

Amyloid proteins : methods and protocols

edited by Einar M. Sigurdsson, Miguel Calero, María Gasset

（Methods in molecular biology / John M. Walker, series editor, 849）（Springer protocols）

Humana Press, c2012

2nd ed

注記

Previous ed.: 2005. Edited by Einar M. Sigurdsson

Includes bibliographical references and index

内容説明・目次

内容説明

Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word "amyloid" indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

目次

Part I. In Vivtro Models and Assays 1. Rapid Generation of Dityrosine Cross-linked A Oligomers via Cu-redox Cycling Adam P. Gunn, Blaine R. Roberts and Ashley I Bush 2. Applications of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins Dahabada H.J. Lopes, Sharmisthia Sinha, Clark Rosenweig, Gal Bitan 3. Preparation of Stable Amyloid -Protein Oligomers of Defined Assembly Order Clark Rosenweig, Kenjiro One, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow 4. Purifications and Fibrillation of Full-Length Recombinant PrP Natallia Makarava and Ilia V. Baskakov 5. Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies Miguel Calero and Maria Gasset 6. Quasielestic Light Scattering Study of Amyloid -Protein Fibrillogenesis Aleksey Lomakin and David B. Teplow 7. Conformations of Microtuble-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer (FRET) Sadasivam Jeganathan, Subashchandrabrose Chinnathamb, Eva-Maria Mandelkow, Echard Mandelkow 8. Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM) Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland 9. X-ray Fibre Diffraction Studies of Amyloid Fibrils Kyle L Morris and Louise C Serpell 10.Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering Annette Eva Langkilde and Bente Vestergaard 11.Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates 12. Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy Susanne Wegmann, Daniel J. Muller and Eckhard Mandelkow 13. Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR Marcal Vilar, Lei Wang and Roland Riek 14.Cyclic Amplification of Prion Protein Misfolding Marcelo A Barria, Dennisse Gonzalez-Romero, and Claudio Soto 15. Search for Amyloid Binding Proteins by Affinity Chromatography Miguel Calero, Agueda Rostagno, and Jorge Ghiso Part II Cell Culture Models and Assays 16. Using Biophysical Approaches for Establishing the Links Between A Aggregation and Cytotoxicity in Vitro Asad Jan and Hilal A. Lashuel 17. Preparation of Cultured Human Vascular Cells Ingvar H. Olafsson, Dadi Th. Vilhjalmsson and Finnbogi R. Thormodsson 18. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse Brain Sebastien A. Gauthier, Susmita Sahoo, Sonia S. Jung, and Efrat Levy 19. In Vitro Assays Measuring Protection by Proteins such as Cystatin C of Primary Cortical Neuronal and Smooth Muscle Cells Sebastein A. Gauthier, Belen Tizon, Susmita Sahoo, and Efrat Levy 20. Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids Carlos Villalobos, Erica Caballero, Sara Sanz-Blasco and Lucia Nunez 21. Bacterial Amyloids Yizhou Zhou, Luz Blanco, Daniel R. Smith, Matthew R. Chapman 22. Study of Amyloids using Yeast Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey and Herman K. Edskes 23. Cell-to-cell Transmission of a-synuclein Aggregate Seung-Jae Lee, Paula Desplats , He-Jin Lee, Brian Spencer and Eliezer Masliah Part III In Vivo Models and Assays 24. Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies Per Westermark 25. Analysis of S100 Oligomers and Amyloids Hugo M. Botelho, Gunter Fritz, Claudio M. Gomes 26. S100A8/A9 Amyloidosis in the Ageing Prostate: Relating ex vivo and in vitro Studies Anna L. Gharibyan, Dina Raveh, Ludmilla A. Morozova-Roche 27. Isolation of Amyloid by Solubilization in Water Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir and Finnbogi R. Thormodsson 28. Histological Staining of Amyloid and Pre-Amyloid Peptides and Proteins in Mouse Tissue Hameetha B. Rajamohamedsait and Einar M. Sigurdsson 29. A Pentameric Luminescent Conjugated Oligothiophene for Optical Imaging of in vitro Formed Amyloid Fibrils and Protein Aggregates in Tissue Sections K. Peter R. Nilsson, Mikael Lindgren and Per Hammarstroem 30. In Vivo Magnetic Resonance Imaging of Amyloid- Plaques in Mice Youssef Zaim Wadghiri, Dung Minh Hoang,, Thomas Wisniewski and Einar M. Sigurdsson 31 The Mouse Model for Scrapie: Inoculation, Clinical Scoring and Histopathological Techniques Michele A. Di Bari, Romolo Nonno and Umberto Agrimi 32. Biochemical Isolation of Insoluble Tau in Transgenic Mouse Models of Tauopathies Carl Julien and Emmanuel Planel 33. Tissue Processing Prior to Analysis of Alzheimer's Disease Associated Proteins and Metabolites, Including Ab Stephen D. Schmidt, Ralph A. Nixon , and Paul M. Mathews 34. Ab Measurement by Enzyme-linked Immunosorbent Assay (ELISA) Stephen D. Schmidt, Matthew J. Mazzella, Ralph A. Nixon, and Paul M. Mathews 35. Cognitive and Sensorimotor Tasks for Assessing Functional Impairments in Mouse Models of Alzheimer's Disease and Related Disorders Allal Boutajangout, Yong Sheng Li, David Quartermain and Einar M Sigurdsson

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詳細情報

• NII書誌ID(NCID): BB13440379
• ISBN:
  • 9781617795503
• LCCN: 2012930138
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 548 p.
• 大きさ: 26 cm
• 分類:
  • DC23 : 616.3995
• 件名:
  • LCSH : Amyloid -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632