Laboratory methods in enzymology: RNA
著者
書誌事項
Laboratory methods in enzymology: RNA
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 530）
Elsevier/Academic Press, 2013
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注記
Includes bibliographical references and indexes
内容説明・目次
内容説明
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
目次
- Computational Prediction of RNA Secondary Structure Reverse-transcription PCR (RT-PCR) Northern blotting Explanatory Chapter: Nuclease Protection Assays In Vitro Transcription from Plasmid or PCR-amplified DNA Measurement of In Vivo RNA Synthesis Rates Method for measuring mRNA decay rate in Saccharomyces cerevisiae Analysis of polysomes from bacteria Polysome Profile Analysis - Yeast Polysome analysis of mammalian cells Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3'
- ends, and poly(A) tails of RNA RNA Radiolabeling Fluorescently Labeling Synthetic RNAs Analysis of RNA by analytical polyacrylamide gel electrophoresis RNA purification by preparative polyacrylamide gel electrophoresis Explanatory Chapter: Nucleic Acid Concentration Determination RNA purification - precipitation methods Reverse Transcriptase Dideoxy Sequencing of RNA RNA Structure Experimental Analysis - Chemical Modification Structural Analysis of RNA Backbone Using In-Line Probing
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