Next-generation MicroRNA expression profiling technology : methods and protocols

著者

    • Fan, Jian-Bing

書誌事項

Next-generation MicroRNA expression profiling technology : methods and protocols

edited by Jian-Bing Fan

（Methods in molecular biology / John M. Walker, series editor, v. 822）（Springer protocols）

Humana Press, c2012

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

目次

1. Controlling miRNA Regulation in Disease Willemijn M. Gommans and Eugene Berezikov 2. Introduction to miRNA Profiling Technologies and Cross-Platform Comparison Sarah Aldridge and James Hadfield 3. Stem-Loop RT-qPCR for MicroRNA Expression Profiling James Hurley, Doug Roberts, Andrew Bond, David Keys, and Caifu Chen 4. Poly(T) Adaptor RT-PCR Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, and Vincent L. Chiang 5. MicroRNA In Situ Hybridization Boye Schnack Nielsen 6. Agilent MicroRNA Microarray Profiling System Petula N. D'Andrade and Stephanie Fulmer-Smentek 7. miRNA Gene Expression Profiling Using Illumina Universal BeadChips Jing Chen, Craig S. April, and Jian-Bing Fan 8. MicroRNA Expression Analysis Using the Affymetrix Platform Suzanne Dee and Robert C. Getts 9. Individualized miRNA Assay Panels Using Optically Encoded Beads Keld Sorensen 10. Microfluidic Primer Extension Assay (MPEA) Markus Beier and Valesca Boisguerin 11. MicroRNA Profiling Using Paraflo Microfluidic Array Technology Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Zhang, Litao Yang, and Xiaolian Gao 12. MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform Shujun Luo 13. Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application Ana Raquel Soares, Patricia M. Pereira, and Manuel A.S. Santos 14. Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing Sam E.V. Linsen and Edwin Cuppen 15. Profiling of Short RNAs Using Helicos Single-Molecule Sequencing Philipp Kapranov, Fatih Ozsolak, and Patrice M. Milos 16. deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data Jian-Hua Yang and Liang-Hu Qu 17. PhenomiR: MicroRNAs in Human Diseases and Biological Processes Andreas Ruepp, Andreas Kowarsch, and Fabian Theis 18. miRNA Expression Profiling: From Reference Genes to Global Mean Normalization Barbara D'haene, Pieter Mestdagh, Jan Hellemans, and Jo Vandesompele 19. miRNA Data Analysis: Next-Gen Sequencing Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, and Arpit Tandon 20. Integrated miRNA Expression Analysis and Target Prediction William Ritchie and John E.J. Rasko 21. miRNAs in Human Cancer Xiaomin Zhong, George Coukos, and Lin Zhang 22. Blood-Based miRNA Preparation for Non-Invasive Biomarker Development Svenja Debey-Pascher, Jing Chen, Thorsten Voss, and Andrea Staratschek-Jox

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