Olfactory receptors : methods and protocols
Author(s)
Bibliographic Information
Olfactory receptors : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 1003）（Springer protocols）
Humana Press, c2013
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist. In Olfactory Receptors: Methods and Protocols, experts in the field contribute chapters that serve to address these challenges. The volume consists of several sections: knowledge dissemination of ORs, theoretical assessments of OR structure and function, as well as development and use of expression systems and experimental functional analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and practical, Olfactory Receptors: Methods and Protocols seeks to aid researchers in furthering the knowledge of olfaction and moving us ever closer to the thrilling discoveries that will follow.
Table of Contents
Part I: Knowledge Dissemination of Olfactory Receptors
1. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors
Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai, Perry L. Miller, Gordon M. Shepherd, and Chiquito J. Crasto
2. HORDE: Comprehensive Resource for Olfactory Receptor Genomics
Tsviya Olender, Noam Nativ, and Doron Lancet
3. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences
Yoshihito Niimura
Part II: Theoretical Assessments of Olfactory Receptor Structure and Function
4. Molecular Modelling of Odorant / Olfactory Receptor Complexes
Landry Charlier, Jeremie Topin, Peter C. Lai, Chiquito J. Crasto, and Jerome Golebiowski
5. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors
Eleanora Alfinito, Jeremy Pousset, and Lino Reggiani
6. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus 'Lock Picks'
Dietmar Krautwurst and Matthias Kotthoff
Part III: Expression Systems for Olfactory Receptors
7. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems
Karolina Corin, Brian Cook, and Shuguang Zhang
8. Human Olfactory Receptors: Recombinant Expression in the Baculovirus / Sf9 Insect Cell System, Functional Characterization, and Odorant Identification
Valery Matarazzo and Catherine Ronin
9. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons
Bettina Malnic
10. Next-Generation Sequencing of the Human Olfactory Receptors
Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, and Nicholas Katsanis
11. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer (BRET)
Guenhael Sanz and Edith Pajot-Augy
12. Olfactory Receptors in Human Airway Epithelia
Xiaoling Gu and Yehuda Ben-Shahar
Part IV: Functional Analysis of Olfactory Receptors
13. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches
Michel Renou and Philippe Lucas
14. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes
Charles W. Luetje, Andrew S. Nichols, Ana Castro, and Benjamin L. Sherman
15. Pharmacology of Mammalian Olfactory Receptors
Richard S. Smith, Zita Peterlin, and Ricardo C. Araneda
16. Visualizing Olfactory Receptor Expression and Localization in Drosophila
Michael Saina and Richard Benton
17. Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods
Kaveh A. Baghaei
Part V: Olfactory Receptor Evolution
18. Evolution of Olfactory Receptors
Kara C. Hoover
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