Plant cell morphogenesis : methods and protocols

著者

• Zarsky, Viktor
• Cvrckova, Fatima

書誌事項

Plant cell morphogenesis : methods and protocols

edited by Viktor Zarsky, Fatima Cvrckova

（Methods in molecular biology / John M. Walker, series editor, 1080）（Springer protocols）

Humana Press, c2014

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on classical light microscopy and histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized electron microscopy techniques, and methods used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by expert researchers in the field and include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

目次

1. Essential Methods of Plant Sample Preparation for Light Microscopy Ales Soukup and Edita Tylova 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy Ales Soukup 3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues Kieran J. D. Lee and J. Paul Knox 4. Automated Microscopy in Forward Genetic Screening of Arabidopsis Tereza Dobisova and Jan Hejatko 5. Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections Jana Albrechtova, Zuzana Kubinova, Ales Soukup, and Jiri Janacek 6. Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells Andreas Nebenfuhr 7. Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM Amparo Rosero, Viktor Zarsky, and Fatima Cvrckova 8. Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces Dorota Kwiatkowska and Agata Burian 9. Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope Olivier Hamant, Pradeep Das, and Agata Burian 10. Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX Pierre Barbier de Reuille, Sarah Robinson, and Richard S. Smith 11. Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy Anne-Lise Routier-Kierzkowska and Richard S. Smith 12. High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy Ichirou Karahara and Byung-Ho Kang 13. Reconstructing Plant Cells in 3D by Serial Section Electron Tomography Kiminori Toyooka and Byung-Ho Kang 14. Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy Jindriska Fiserova and Martin W. Goldberg 15. Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections Ilse Foissner and Margit Hoeftberger 16. Live Cell Imaging of Arabidopsis Root Hairs Tijs Ketelaar 17. Morphological Analysis of Cell Growth Mutants in Physcomitrella Luis Vidali and Jeffrey P. Bibeau 18. Plant Cell Lines in Cell Morphogenesis Research Daniela Seifertova, Petr Klima, Marketa Parezova, Jan Petrasek, Eva Zazimalova, and Zdenek Opatrny 19. Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes Radek Bezvoda, Roman Pleskot, Viktor Zarsky, and Martin Potocky 20. Lab-on-a-Chip for Studying Growing Pollen Tubes Carlos G. Agudelo, Muthukumaran Packirisamy, and Anja Geitmann 21. Laser Microdissection of Plant Cells Yvonne Ludwig and Frank Hochholdinger 22. Optical Trapping in Plant Cells Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren 23. Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery Fatima Cvrckova and Michal Hala

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詳細情報

• NII書誌ID(NCID): BB14348429
• ISBN:
  • 9781627036429
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 285 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632