Circulating microRNAs : methods and protocols
著者
書誌事項
Circulating microRNAs : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 1024）（Springer protocols）
Humana Press, c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
目次
1. Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research
Nobuyoshi Kosaka, Yusuke Yoshioka, Keitaro Hagiwara, Naoomi Tominaga and Takahiro Ochiya
2. Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture
Takayuki Kogure and Tushar Patel
3. Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells
Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morell
4. Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse
Carolina Villarroya-Beltri, Cristina Gutierrez-Vazquez, Francisco Sanchez-Madrid, and Maria Mittelbrunn
5. Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo
Frederik J Verweij, Monique AJ van Eijndhoven, Jaap Middeldorp and D Michiel Pegtel
6. Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells
Tian Sheng Chen and Sai Kiang Lim
7. Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction
Steven J. Greco, Pranela Rameshwar
8. Isolation of Circulating MicroRNA Associated with RNA-binding Protein
Andrey Turchinovich, Ludmila Weiz, and Barbara Burwinkel
9. Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids
Cecilia Lasser
10. Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR
Leni Moldovan, Kara Batte, Yijie Wang, Jon Wisler, and Melissa Piper
11. Direct Serum Assay for MicroRNA in Cancer Patients
Sota Asaga and Dave S.B. Hoon
12. Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ
Victoria N. Parikh and Stephen Y. Chan
13. Analysis of Circulating MicroRNA by Microarray in Liver Disease
Yoshiki Murakami and Toshihito Tanahashi
14. Isolation of Circulating MicroRNA in Saliva
Alessia Gallo, Ilias Alevizos
15. Purification of RNA From Milk Whey
Hirohisa Izumi, Nobuyoshi Kosaka, Takashi Shimizu, Kazunori Sekine, Takahiro Ochiya, and Mitsunori Takase
16. Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases
Akira Machida, Takuya Ohkubo, and Takanori Yokota
17. Methods of MicroRNA Quantification in Urinary Sediment
Wang Gang, and Szeto Cheuk Chun,
18. Circulating microRNA for the Identification of Forensically Relevant Body Fluids
Erin K. Hanson and Jack Ballantyne
19. Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease
Luke A. Selth, Scott Townley, Joanna Gillis, Wayne Tilley and Lisa Butler
20. A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma
Makiko Ichikawa and Hideo Akiyama
21. Nanopore Single-molecule Detection of Circulating microRNAs
Li-Qun Gu and Yong Wang
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