MicroRNA and cancer : methods and protocols
Author(s)
Bibliographic Information
MicroRNA and cancer : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 676）（Springer protocols）
Humana Press, c2011
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents:
* Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment
* Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study
* Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs
* Cross-cited notes on troubleshooting and avoiding known pitfalls
Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.
Table of Contents
Part I: Reviews
1. MicroRNA Biogenesis and Cancer
Julia Winter and Sven Diederichs
2. Computational Identification of MiRNAs Involved in Cancer
Anastasis Oulas, Nestoras Karathanasis, and Panayiota Poirazi
3. The Principles of MiRNA-Masking Antisense Oligonucleotides Technology
Zhiguo Wang
4. The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology
Zhiguo Wang
5. Modulation of MicroRNAs for Potential Cancer Therapeutics
Wei Wu
Part II: Protocols
6. Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In situ Hybridization
Ashim Gupta and Yin-Yuan Mo
7. MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference
Julia Winter and Sven Diederichs
8. MiRNA Profiling on High Throughput OpenArray (TM) System
Elena V. Grigorenko, Elen Ortenberg, James Hurley, Andrew Bond, and Kevin Munnelly
9. Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs
Guo-Jun Zhang
10. High Throughput and Reliable Protocols for Animal microRNA Library Cloning
Caide Xiao
11. MicroRNA Regulation of Growth Factor Receptor Signalling in Human Cancer Cells
Keith M. Giles, Andrew Barker, Priscilla M. Zhang, Michael R. Epis, and Peter J. Leedman
12. Epigenetic Regulation of MicroRNA Expression in Cancer
Hani Choudhry and James W.F. Catto
13. In vitro Functional Study of miR-126 in Leukemia
Zejuan Li and Jianjun Chen
14. Prediction of the Biological Effect of Polymorphisms within MicroRNA Binding Sites
Debora Landi, Roberto Barale, Federica Gemignani, and Stefano Landi
15. The Guideline of the Design and Validation of MiRNA Mimics
Zhiguo Wang
16. Analysis of Targets and Functions Co-Regulated by MicroRNAs
Shu-Jen Chen and Hua-Chien Chen
17. Utilization of SSCprofiler to Predict a New MiRNA Gene
Anastasis Oulas and Panayiota Poirazi
18. MicroRNA-Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing
Thomas Streichert, Benjamin Otto, and Ulrich Lehmann
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