Analyzing microbes : manual of molecular biology techniques
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書誌事項
Analyzing microbes : manual of molecular biology techniques
（Springer protocols）
Springer, c2013
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注記
Includes bibliographical references and index
収録内容
- Microbial DNA extraction, purification, and quantitation
- Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, and Dilip K. Arora
- Fluorescent-based detection, quantitation, and expression of viral gene by qRT-PCR
- Shelly Praveen and Vikas Koundal
- Restriction enzymes and their role in microbiology
- Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, and Dilip K. Arora
- Genetic fingerprinting techniques for molecular characterisation of microbes
- Annette Reineke and K. Uma Devi
- Agarose gel electrophoresis and polyacrylamide gel electrophoresis : methods and principles
- Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, and Dilip K. Arora
- Molecular identification of microbes : I. Macrophomina phaseolina
- Bandamaravuri Kishore Babu, T. Kiran Babu, and Rajan Sharma
- Molecular identification of microbes : II. Bacillus
- Anil Kumar Saxena
- Molecular identification of microbes : III. Pseudomonas
- Bhim Pratap Singh and Ratul Saikia
- Molecular identification of microbes : IV. Vibrio
- Hirak Ranjan Dash, Neelam Mangwani, and Surajit Das
- Molecular identification of microbes : V. methylotrophs
- Kamlesh K. Meena, Manish Kumar, and D.P. Singh
- Preservation and maintenance of microbial cultures
- Sudheer Kumar ... [et al.]
- Microbes from extreme environment : molecular identification procedures
- Surajit Das
- ELISA-based identification and detection of microbes
- Jyoti Verma, Sangeeta Saxena, and Sunil G. Babu
- Analysis of microbial diversity and construction of metagenomic library
- Thangamani Rajesh, Jeyaprakash Rajendhran, and Paramasamy Gunasekaran
- Bioinformatics tools for interpretation of data used in molecular identification
- Suchi Smita ... [et al.]
- Molecular phylogenetics of microbes
- Surajit Das and Hirak Ranjan Dash
- Microarray technology : basic concept, protocols, and applications
- P.P. Dubey and Dinesh Kumar
- Microarray analysis of different functional genes of microorganisms
- Hirak Ranjan Dash and Surajit Das
- DNA cloning and sequencing
- Bandamaravuri Kishore Babu, Anu Sharma, and Hari Kishan Sudini
- Biological sequence analysis : algorithms and statistical methods
- Suchi Smita ... [et al.]
- Appendix 1
- Biosafety, GLP, and biosecurity
- Appendix 2
- Address for instruments and chemicals suppliers
内容説明・目次
内容説明
This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.
目次
Microbial DNA extraction, purification and quantitation.- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR.- Restriction enzymes and their role in microbiology.- Genetic fingerprinting techniques for molecular characterization of microbes.- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles.- Molecular identification of microbes.- Preservation and maintenance of microbial cultures.- Microbes from extreme environment: Molecular identification procedures.- ELISA based identification and detection of microbes.- Analysis of microbial diversity and construction of metagenomic library.- Bioinformatics tools for interpretation of data used in molecular identification.- Molecular phylogenetics of microbes.- Microarray technology: Basic concept, protocols and applications.- Microarray analysis of different functional genes of microorganisms.- DNA cloning and sequencing.- Biological sequence analysis: Algorithms and statistical methods.
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