Nanomaterial Interfaces in Biology : Methods and Protocols
著者
書誌事項
Nanomaterial Interfaces in Biology : Methods and Protocols
（Methods in molecular biology / John M. Walker, series editor, 1025）（Springer protocols）
Humana Press : Springer, c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.
目次
Part I. Making Bio-Nano Interfaces
1. Preparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications
Daniel F. Moyano, Bradley Duncan, and Vincent M. Rotello
2. DNA Conjugation to Nanoparticles
Sunho Park
3. Conjugation of Nanoparticles to Proteins
Marie-Eve Aubin-Tam
4. Water-Solubilization and Functionalization of Semiconductor Quantum Dots
Christina M. Tyrakowski, Adela Isovic, and Preston T. Snee
5. Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates
W. Russ Algar, Juan B. Blanco-Canosa, Rachel L. Manthe, Kimihiro Susumu, Michael H. Stewart, Philip E. Dawson, and Igor L. Medintz
6. Reliable Methods for Silica Coating of Au Nanoparticles
Isabel Pastoriza-Santos and Luis M. Liz-Marzan
7. Surface Modifications by Polymers for Biomolecule Conjugation
Laura Sola, Marina Cretich, Francesco Damin, and Marcella Chiari
8. Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors
Franscesca Frascella and Carlo Ricciardi
Part II. Characterizing Bio-Nano Interfaces
9. Stability and Aggregation Assays of Nanoparticles in Biological Media
James Kah
10. Electrochemical Measurements of DNA Melting on Surfaces
Irina Belozerova, Dongbiao Ge, and Rastislav Levickyy
11. Formation and Characterization of Nanoparticle-Protein Corona
Marco P. Monopoli, Andrzej S. Pitek, Iseult Lynch, and Kenneth A. Dawson
12. Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution
Hyeran Noh, Naris Barnthip, Purnendu Parhi, and Erwin A. Vogler
13. Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment
Christin Grabinski, John Schlager, and Saber Hussain
14. Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles
Roberto Marotta, A. Falqui, A. Curcio, A. Quarta, and Teresa Pellegrino
Part III. Implementing Bio-Nano Interfaces
15. Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption
Zaki G. Estephan and Joseph B. Schlenoff
16. Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles
Xi-Jun Chen, Robert J. Hickey, and So-Jung Park
17. Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging
Taku Cowger and Jin Xie
18. Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy
Daniel Montiel, Emma V. Yates, Li Sun, Marissa M. Sampias, John Malona, Eirk J. Sorensen, and Haw Yang
19. Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs
Tennyson Doane and Clemens Burda
20. Modification of Carbon Nanotubes for Gene Delivery Vectors
Victor Ramos-Perez, Anna Cifuentes, Nuria Coronas, Ana de Pablo, and Salvador Borros
21. Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors
Daniele Pezzoli, Anna Kajaste-Rudnitski, Roberto Chiesa, and Gabriele Candiani
22. Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation
Joan Comenge and Victor F. Puntes
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