Genotoxicity assessment : methods and protocols
著者
書誌事項
Genotoxicity assessment : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1044）（Springer protocols）
Humana Press, c2013
- : [hardcover]
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.
目次
- Part I: Gene Mutation Assays 1. Bacterial Mutation Assays Errol Zeiger 2. In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi 3. Erythrocyte--Based Pig--a Gene Mutation Assay Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger 4. Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich 5. In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich Part II
- Assays for Chromosomal Abnormalities 6. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti 7. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse Francesca Pacchierotti and Valentina Stocchi 8. Chromosomal Aberration Test in Human Lymphocytes Christian Johannes and Guenter Obe 9. In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood Sawako Kasamoto, Shoji Masumori, and Makoto Hayashi 10. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells Claudia Bolognesi and Michael Fenech 11. Flow Cytometric Determination of Micronucleus Frequency Azeddine Elhajouji and Magdalena Lukamowicz-Rajska 12. Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test Ilse Decordier and Micheline Kirsch-Volders 13. Comparative Genomic Hybridization (CGH) Genotoxicology Baumgartner A 14. The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity Bella B. Manshian, Neenu Singh, and Shareen H. Doak Part III: Tests for Primary DNA Damage 15. g--H2AX Detection in Somatic and Germ Cells of Mice Eugenia Cordelli and Lorena Paris 16. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers Julian Laubenthal, Michal R. Gdula , Alok Dhawan, and Diana Anderson 17. The Comet Assay: Assessment of in vitro and in vivo DNA Damage Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan 18. The Comet Assay in Human Biomonitoring Diana Anderson, Alok Dhawan, and Julian Laubenthal 19. The Comet assay in Marine Animals G. Frenzilli and B.P. Lyons 20. Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo Fabrice Nesslany 21. 32P--Postlabeling Analysis of DNA Adducts Heinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt Part IV: Assays in Plants and Aleternate Animal Models 22. Micronucleus Assay with Tetrad Cells of Tradescantia Miroslav Misik, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller 23. The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila Ricard Marcos and Erico R. Carmona Part V: Guidelines 24. Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH) Gireesh Kamath H. and K.S. Rao
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