- Volume
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pt. A ISBN 9780124200708
Description
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.
This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:
Pulse-chase analysis to measure protein degradation
Labeling a protein with fluorophores using NHS ester derivitization
Immunoaffinity purification of proteins
Proteolytic affinity tag cleavage
Purification of GST-tagged proteins
Table of Contents
Practical Steady-state Enzyme Kinetics
Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
Preparation of protein samples for mass spectrometry and N-terminal sequencing
Lysis of mammalian and Sf9 cells
In Vivo [35 S]-Methionine Incorporation
Pulse-chase analysis to measure protein degradation
Labeling of a Protein with Fluorophores using Maleimide Derivitization
Labeling a protein with fluorophores using NHS ester derivitization
Protein Derivitization-Expressed Protein Ligation
Protein Biotinylation
Purification of His-tagged proteins
Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
Immunoaffinity purification of proteins
Affinity Purification of Protein Complexes Using TAP Tags
Strep-tagged protein purification
Proteolytic affinity tag cleavage
Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Purification of GST-tagged proteins
- Volume
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pt. C ISBN 9780124201194
Description
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
Table of Contents
TCA PrecipitationCoimmunoprecipitation of Proteins from YeastCoupling Antibody to Cyanogen Bromide-Activated SepharoseAnalysis of Protein-Protein Interactions by CoimmunoprecipitationUse and Application of Hydrophobic Interaction Chromatography for Protein PurificationHydroxyapatite Chromatography: Purification Strategies for Recombinant ProteinsSalting out of Proteins Using Ammonium Sulfate PrecipitationUsing Ion Exchange Chromatography to Purify a Recombinantly Expressed ProteinGel Filtration Chromatography (Size Exclusion Chromatography) of ProteinsExpression and Purification of Membrane ProteinsExplanatory Chapter: Choosing the Right DetergentOne-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)Coomassie Blue StainingSilver Staining of SDS-polyacrylamide GelStandard In Vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA BindingProtein Filter BindingExplanatory Chapter: Troubleshooting recombinant protein expression: generalExplanatory Chapter: Troubleshooting protein expression: what to do when the protein is not solubleWestern Blotting using Chemiluminescent Substrates
- Volume
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pt. B ISBN 9780124201200
Description
Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.
Table of Contents
Small-scale expression of proteins in E. coli Protein expression-Yeast Recombinant protein expression in baculovirus-infected insect cells Single cell cloning of a stable mammalian cell line In vitro synthesis of proteins in bacterial extracts Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation Yeast two-hybrid screen UV cross-linking of interacting RNA and protein in cultured cells Analysis of RNA-protein interactions by cell mixing General protein-protein cross-linking Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system Dissecting a known RNA-protein interaction using a yeast three-hybrid system Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system
- Volume
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pt. D ISBN 9780128002797
Description
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.
In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts.
Table of Contents
1. Purification of His-tagged ProteinsAnne Spriestersbach, Jan Kubicek, Frank Schafer, Helena Block and Barbara Maertens2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) TagKrisna C. Duong-Ly and Sandra B. Gabelli3. Immunoaffinity Purification of ProteinsJennifer M. Kavran and Daniel J. Leahy4. Affinity Purification of Protein Complexes Using TAP TagsErica Gerace and Danesh Moazed5. Strep-tagged Protein PurificationBarbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schafer6. Proteolytic Affinity Tag CleavageHelena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schafer7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose BeadsErica Gerace and Danesh Moazed8. Protein Affinity Purification using Intein/Chitin Binding Protein TagsSarah F. Mitchell and Jon R. Lorsch9. Purification of GST-tagged ProteinsFrank Schafer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek
by "Nielsen BookData"
