Stem cell niche : methods and protocols
著者
書誌事項
Stem cell niche : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1035）（Springer protocols）
Humana Press, c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Interest in a specialized microenvironment or "niche" regulating hemopoietic stem cell function has been steadily growing since the idea was first proposed by Ray Schofield over three decades ago. This growing interest, as well as more recently the interest in cellular-molecular-biochemical characterization of not only the hemopoietic stem cell niche but the niches for other stem cells, incited the compilation of Stem Cell Niche: Methods and Protocols. In this volume, scientists have provided protocols that will provide both a flavor of the field and hopefully stimulate new approaches and methodologies by those interested in the stem cell niche. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Stem Cell Niche: Methods and Protocols seeks to serve both experts and novices in the stem cell field with well-established protocols on this exciting subject.
目次
1. Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries
Lichao Luo, Phing Chian Chai, and Yu Cai
2. Identification of Male and Female Germline Stem Cells in Drosophila
Shree Ram Singh, Madhuri Kango-Singh, and Eviatar Nevo
3. Visualization of Adult Stem Cells Within their Niches Using the Drosophila Germline as a Model System
Annekatrin Koenig and Halyna R. Shcherbata
4. Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates
Paulo Henrique Almeida Campos-Junior, Guilherme Mattos Jardim Costa, Gleide Fernandes de Avelar, Tania Mara Segatelli, Samyra Maria Santos Nassif Lacerda, Pedro Manuel Aponte, and Luiz Renato de Franca
5. In vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche
Brahmananda Reddy Chitteti, Monique Bethel, Sherry L. Voytik-Harbin, Melissa A. Kacena, and Edward F. Srour
6. Isolation of Embryonic Hematopoietic Niche Cells by Flow Cytometry and Laser Capture Micro-Dissection
Daisuke Sugiyama and Tatsuya Sasaki
7. Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marrow
Emma L. Williams, Kate White, and Richard O.C. Oreffo
8. Primary Marrow Derived Stromal Cells: Isolation and Manipulation
Aravind Ramakrishnan, Beverly Torok-Storb, and Manoj M. Pillai
9. Detection In vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogenic Differentiation and Maturation of Stromal Stem Cell
Igor A. Khlusov, Natalya M. Shevtsova, and Marina Yu
10. The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting
Yi Shen, Brenda Williams, Melonie J. Storan, and Susan K. Nilsson
11. Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minipump System
Maria Victoria Gomez Gaviro, Pedro Luis Sanchez Fernandez, Robin Lovell Badge, and Francisco Fernandez Aviles
12. Unbiased Stereological Method to Assess Proliferation Throughout the Subependymal Zone
Ana Mendanha Falcao, Joana Almeida Palha, Ana Catarina Ferreira, Fernanda Marques, Nuno Sousa and Joao Carlos Sousa
13. Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells
Paras Kumar Mishra, Nicholas John Kuypers , Shree Ram Singh, Leiberh Noel Diaz , Vishalakshi Chavali, and Suresh C. Tyagi
14. Human and Murine Skeletal Muscle Reserve Cells
Rana Abou-Khalil, Fabien Le Grand, and Benedicte Chazaud
15. Modulation of the Host Skeletal Muscle Niche for Donor Satellite Cell Grafting
Luisa Boldrin and Jennifer E. Morgan
16. Isolation of ckit+ Human Amniotic Fluid Stem Cells from Second Trimester
Michela Pozzobon, Martina Piccoli, Andrea Alex Schiavo, Anthony Atala, and Paolo De Coppi
17. Hypoxia and Visualization of the Stem Cell Niche
Pramudita R Ali Dalloul
18. Detection and Isolation of Human Disseminated Tumor Cells in the Murine Bone Marrow Stem Cell Niche
Yusuke Shiozawa, Russell S. Taichman, and Evan T. Keller
19. Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patients with Acute Myeloid Leukemia
Van T. Hoang, Isabel Taubert, Karina Borowski, Abraham Zepeda-Moreno, Dan Ran, Eike C. Buss, Patrick Wuchter, Volker Eckstein, and Anthony D. Ho
20. Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension
Heather A. McCauley and Geraldine Guasch
21. Isolation and Propagation of Colon Cancer Stem Cells
Pramudita R. Prasetyanti, Cheryl Zimberlin, Felipe De Sousa E. Melo, and Jan Paul Medema
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