miRNA maturation : methods and protocols
著者
書誌事項
miRNA maturation : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1095）（Springer protocols）
Humana Press, c2014
大学図書館所蔵 全1件
注記
Includes bibliographical references and index
内容説明・目次
内容説明
In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
目次
Part I
1. The Pathway of miRNA Maturation
Michael Sand
2. Micro RNA Maturation and Human Disease
Marlen Hesse and Christoph Arenz
3. Approaches to the Modulation of miRNA Maturation
Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young
Part II
4. Expression Profiling of Components of the miRNA Maturation Machinery
Michael Sand and Marina Skrygan
5. Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8
Ian Barr and Feng Guo
6. In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity
Vera Bilan, Danilo Allegra, Florian Kuchenbauer, Daniel Mertens
7. Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates
Marlen Hesse, Brian P. Davies and Christoph Arenz
8. A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer
Eileen Magbanua and Ulrich Hahn
9. Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification
Saskia Neubacher and Christoph Arenz
10. Identification of Inhibitors of microRNA Function from Small Molecule Screens
Colleen M. Connelly and Alexander Deiters
11. Quantitative RT-PCR Specific for Precursor and Mature miRNAs
Hannah Zoellner, Stephan A. Hahn and Abdelouahid Maghnouj.
12. Cellular MicroRNA Sensors Based on Luciferase Reporters
Colleen M. Connelly and Alexander Deiters
13. Inhibition of miRNA maturation by Peptide Nucleic Acids
Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari and Alessandra Romanelli
14. Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs
Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, and Roberto Gambari
15. Lentiviral Overexpression of miRNAs
Hannah Zoellner, Stephan A. Hahn and Abdelouahid Maghnouj
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