miRNA maturation : methods and protocols

著者

• Arenz, Christoph

書誌事項

miRNA maturation : methods and protocols

edited by Christoph Arenz

（Methods in molecular biology / John M. Walker, series editor, 1095）（Springer protocols）

Humana Press, c2014

注記

Includes bibliographical references and index

内容説明・目次

内容説明

In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.

目次

Part I 1. The Pathway of miRNA Maturation Michael Sand 2. Micro RNA Maturation and Human Disease Marlen Hesse and Christoph Arenz 3. Approaches to the Modulation of miRNA Maturation Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young Part II 4. Expression Profiling of Components of the miRNA Maturation Machinery Michael Sand and Marina Skrygan 5. Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8 Ian Barr and Feng Guo 6. In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity Vera Bilan, Danilo Allegra, Florian Kuchenbauer, Daniel Mertens 7. Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates Marlen Hesse, Brian P. Davies and Christoph Arenz 8. A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer Eileen Magbanua and Ulrich Hahn 9. Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification Saskia Neubacher and Christoph Arenz 10. Identification of Inhibitors of microRNA Function from Small Molecule Screens Colleen M. Connelly and Alexander Deiters 11. Quantitative RT-PCR Specific for Precursor and Mature miRNAs Hannah Zoellner, Stephan A. Hahn and Abdelouahid Maghnouj. 12. Cellular MicroRNA Sensors Based on Luciferase Reporters Colleen M. Connelly and Alexander Deiters 13. Inhibition of miRNA maturation by Peptide Nucleic Acids Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari and Alessandra Romanelli 14. Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, and Roberto Gambari 15. Lentiviral Overexpression of miRNAs Hannah Zoellner, Stephan A. Hahn and Abdelouahid Maghnouj

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詳細情報

• NII書誌ID(NCID): BB15197500
• ISBN:
  • 9781627037020
• LCCN: 2013949785
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: x, 192 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.88
• 件名:
  • LCSH : RNA -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632