The inflammasome : methods and protocols

著者

    • De Nardo, Christine
    • Latz, Eicke

書誌事項

The inflammasome : methods and protocols

edited by Christine M. De Nardo and Eicke Latz

(Methods in molecular biology / John M. Walker, series editor, 1040)(Springer protocols)

Humana Press, c2013

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This Methods in Molecular Biology book offers methods for studying inflammasome function, including generation of inflammasome stimuli, monitoring of caspase-1 activity and processing, activation of IL-1 cytokines, plus lab protocols, material lists and tips.

目次

1. Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia Mareike Schnaars, Hannes Beckert, and Annett Halle 2. Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP Clara Westwell-Roper, Aisling Dunne, Man-Lyang Kim, C. Bruce Verchere, and Seth L. Masters 3. Assessment and Quantification of Crystal-induced Lysosomal Damage Peter Duewell and Eicke Latz 4. Assessment of Inflammasome Activation in Primary Human Immune Cells Theo S. Plantinga, Leo A.B. Joosten, and Mihai G. Netea 5. NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants Marie Yang, Claire H.A. Hearnden, Ewa Oleszycka and Ed C. Lavelle 6. Measuring Inflammasome Activation in Response to Bacterial Infection Petr Broz and Denise M. Monack 7. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity Manira Rayamajhi, Yue Zhang, and Edward A. Miao 8. ASC Speck Formation as a Read-out for Inflammasome Activation Andrea Stutz, Gabor L. Horvath, Brian G. Monks, and Eicke Latz 9. Immunoblotting for Active Caspase-1 Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind and Veit Hornung 10. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1 , IL-1 and Caspase-1 Katharina S. Schneider1, Christina J. Thomas, and Olaf Gross 11. Reconstituting the NLRP1 Inflammasome in vitro Benjamin Faustin and John C. Reed 12. Assessing ATP Binding and Hydrolysis by NLR Proteins Jinyao Mo and Joseph A. Duncan 13. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition Eric M. Kofoed and Russell E. Vance 14. Analysis of Microbiota Alterations in Inflammasome-deficient Mice Eran Elinav, Christoph A Thaiss, and Richard A Flavell 15. Quantification of Adipose Tissue Leukocytosis in Obesity Ryan Grant, Yun-Hee Youm, Anthony Ravussin, and Vishwa Deep Dixit 16. In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates Tamiko Yanagida, Kaoru Orihashi and Hajime Kono

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