Myogenesis : methods and protocols

著者

• DiMario, Joseph X.

書誌事項

Myogenesis : methods and protocols

edited by Joseph X. DiMario

（Methods in molecular biology / John M. Walker, series editor, 798）（Springer protocols）

Humana Press : Springer, c2012

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology. Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells. Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume. Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.

目次

Part I: Isolation and Analysis of Skeletal Muscle Precursor Cells 1. Isolation and Characterization of Human Fetal Myoblasts Ariya D. Lapan and Emanuela Gussoni 2. Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System Maria Elena Danoviz and Zipora Yablonka-Reuveni 3. Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki 4. Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, and Maurilio Sampaolesi 5. Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario 6. Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya 7. Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm Sara J. Venters and Charles P. Ordahl Part II: Non-Mammalian Models of Myogenesis 8. Analysis of Skeletal Muscle Development in Drosophila Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan, and Richard M. Cripps 9. Immunocytochemistry to Study Myogenesis in Zebrafish Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto 10. Immunofluorescent Localization of Proteins in C. elegans Muscle Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian Part III: Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse 11. Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle Stephen E. Alway and Robert G. Cutlip 12. Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading Sue C. Bodine and Keith Baar 13. Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size Chia-Ling Wu and Susan C. Kandarian 14. In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique Marta L. Fiorotto, Horacio A. Sosa, Jr., and Teresa A. Davis Part IV: Generation of Viral Vectors and Transgenic Mice 15. Recombinant Adeno-Associated Viral Vector Production and Purification Jin-Hong Shin, Yongping Yue, and Dongsheng Duan 16. Generation of Lentiviral Vectors for Use in Skeletal Muscle Research Christophe Pichavant and Jacques P. Tremblay 17. Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice Christoph Lepper and Chen-Ming Fan Part V: Muscle Profiling 18. Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar 19. Analysis of Lipid Profiles in Skeletal Muscles Vassilis Mougios and Anatoli Petridou 20. Proteomic Analysis of Dystrophic Muscle Caroline Lewis, Philip Doran, and Kay Ohlendieck Part VI: Experimental Approaches in Calcium Imaging of Skeletal Muscle 21. Detection of Calcium Release via Ryanodine Receptors Jerry P. Eu and Gerhard Meissner 22. Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle Mariana Casas, Francisco Altamirano, and Enrique Jaimovich 23. Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers Noah Weisleder, Jingsong Zhou, and Jianjie Ma 24. Analysis of Calcium Transients in Cardiac Myocytes and Assessment of the Sarcoplasmic Reticulum Ca2+-ATPase Contribution Anand Mohan Prasad and Giuseppe Inesi Part VII: Analysis of Gene Promoter Transcriptional Activity 25. Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells Charis L. Himeda, Phillip W.L. Tai, and Stephen D. Hauschka 26. Analysis of Fiber Type Differences in Reporter Gene Expression of b-Gal Transgenic Muscle Phillip W.L. Tai, Catherine L. Smith, John C. Angello, and Stephen D. Hauschka 27. Determination of Gene Promoter Activity in Skeletal Muscles In Vivo Sarah M. Senf and Andrew R. Judge Part VIII: RNA-Mediated Gene Regulation 28. Determination of MiRNA Targets in Skeletal Muscle Cells Zhan-Peng Huang, Ramon Espinoza-Lewis, and Da-Zhi Wang 29. shRNA-Mediated Gene Knockdown in Skeletal Muscle Muriel Golzio, Jean-Michel Escoffre, and Justin Teissie Part IX: Analysis of Protein-DNA Interactions 30. Detection of NF-kB Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis Jason M. Dahlman and Denis C. Guttridge 31. Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin Immunoprecipitation Assays Yasuyuki Ohkawa, Chandrashekara Mallappa, Caroline S. Dacwag Vallaster, and Anthony N. Imbalzano 32. An Improved Restriction Enzyme Accessibility Assay (REAA) for Analyzing Changes in Chromatin Structure in Samples of Limited Cell Number Yasuyuki Ohkawa, Chandrashekara Mallappa, Caroline S. Dacwag Vallaster, and Anthony N. Imbalzano 33. ChIP Enriched in Silico Targets (ChEST), a ChIP on Chip Approach Applied to Analyzing Skeletal Muscle Genes Guillaume Junion and Krzysztof Jagla

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詳細情報

• NII書誌ID(NCID): BB15447200
• ISBN:
  • 9781617793424
• LCCN: 2011939748
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 558 p.
• 大きさ: 27 cm
• 分類:
  • LCC : QL979
  • DC23 : 571.86
• 件名:
  • LCSH : Myogenesis
  • MESH : Muscle Development -- Laboratory Manuals
  • FREE : Aufsatzsammlung
• 親書誌ID:
  • BA10794673
  • BA88873632