Flavins and flavoproteins : methods and protocols
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Bibliographic Information
Flavins and flavoproteins : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1146）（Springer protocols）
Humana Press, c2014
- : hbk.
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Includes bibliographical references and index
Description and Table of Contents
Description
In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.
Table of Contents
Part I
1. Structure and General Properties of Flavins
Ana Maria Edwards
2. Recent advances in Riboflavin Biosynthesis
Ilka Haase, Tobias Grawert, Boris Illarionov, Adelbert Bacher and Markus Fischer
3. Natural Riboflavin Analogs
Danielle Biscaro Pedrolli, Frank Jankowitsch, Julia Schwarz, Simone Langer, Shinobu Nakanishi and Matthias Mack
4. A Roadmap to the Isotopolog Space of Flavocoenzymes
Adelbert Bacher, Boris Illarionov, Wolfgang Eisenreich and Markus Fischer
5. Electron Transferases
Patricia Ferreira, Marta Martinez-Julvez and Milagros Medina
6. Aldonolactone Oxidoreductases
Nicole G.H. Leferink and Willem J.H. van Berkel
7. Flavins and Flavoproteins: Applications in Medicine
Esther Jortzik, Lihui Wang, Jipeng Ma and Katja Becker
Part II
8. Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms
Christopher R. Pudney, Sam Hay and Nigel S. Scrutton
9. On the in vivo Redox State of Flavin-Containing Photosensory Receptor Proteins
Aleksandra Bury and Klaas J. Hellingwerf
10. Computational Spectroscopy, Dynamics, and Photochemistry of Photosensory Flavoproteins
Tatiana Domratcheva, Anik Udvarhelyi, and Abdul Rehaman Moughal Shahi
11. NMR Spectroscopy on Flavins and Flavoproteins
Franz Muller
12. Solid-State NMR of Flavins and Flavoproteins
Anne-Frances Miller
13. EPR on Flavoproteins
Richard Brosi, Robert Bittl, and Christopher Engelhard
14. FTIR Spectroscopy of Flavin-Binding Photoreceptors
Daichi Yamada and Hideki Kandori
15. Resonance Raman Spectroscopy
Jiang Li and Teizo Kitagawa
16. Photoactivation Mechanisms of Flavin-binding Photoreceptors Revealed through Ultrafast Spectroscopy and Global Analysis Methods
Tilo Mathes, Ivo H.M. van Stokkum and John T.M. Kennis
17. Excited State Chemistry of Flavoproteins
Raymond F. Pauszek, Goutham Kodali, and Robert J. Stanley
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