Confocal microscopy : methods and protocols
著者
書誌事項
Confocal microscopy : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1075）（Springer protocols）
Humana Press, c2014
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.
目次
1. Reflecting on Confocal Microscopy: A Personal Perspective
John White
2. Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques
Stephen W. Paddock and Kevin W. Eliceiri
3. Confocal Microscopy on the Internet
Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko, Anna P. Ozarowska, Adam M. Rainey and Michael W. Davidson
4. Using Photoshop with Images Created by a Confocal System
Jerry Sedgewick
5. Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy
Timo Zimmermann, Joanne Marrison, Karen Hogg, and Peter O'Toole
6. Low Magnification Confocal Microscopy of Tumor Angiogenesis
George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster, Yiwen Li, and Rong Wen
7. Confocal Imaging of Butterfly Tissue
Craig R. Brunetti
8. Confocal Microscopy of Cardiac Myocytes
Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, and Louis Terracio
9. Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction
Ian P. Coyle
10. Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens
Angela V. Klaus, Valerie Schawaroch, and Kevin J. Frischmann
11. Vital Imaging of Multicellular Spheroids
Paulo J. Oliveira, Ed Perkins, and Jon Holy
12. Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos
Barbara Fasulo and William Sullivan
13. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells
Kevin F. O'Connell and Andy Golden
14. Measurement in the Confocal Microscope
Guy Cox
15. A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung
Robert L. Conhaim, Dennis M. Heisey, and Greg E. Leverson
16. Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney
Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini, and John F. Bertram
17. Evaluating Confocal Microscopy System Performance
Robert M. Zucker
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