Developmental biology of the sea urchin and other marine invertebrates : methods and protocols
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Bibliographic Information
Developmental biology of the sea urchin and other marine invertebrates : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1128）（Springer protocols）
Humana Press, c2014
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Includes bibliographical references and index
Description and Table of Contents
Description
In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.
Table of Contents
1. Culturing Larvae of Marine Invertebrates
Richard R. Strathmann
2. Obtaining Gametes and Embryos of Ascidians
Charles C. Lambert
3. Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis
Anthony Pires
4. Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential
Andreas Heyland, Roger Croll, Sophie Goodall, Jeff Kranyak, and Russell Wyeth
5. Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses
Michael H. Temkin
6. An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images
George von Dassow
7. TEM Analyses of Chaetognath Reproductive Organs
George L. Shinn
8. SEM Analysis of Marine Invertebrate Gametes
John Buckland-Nicks
9. Imaging Neural Development in Embryonic and Larval Sea Urchins
Oliver Krupke, Shunsuke Yaguchi, Junko Yaguchi, and Robert D. Burke
10. Preparation and Use of Sea Urchin Egg HomogenatesAnthony J. Morgan & Antony Galione
11. Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata
Alex McDougall, Karen Wing-man Lee and Remi Dumollard
12. Isolating Specific Embryonic Cells of the Sea Urchin by FACS
Celina Juliano, S. Zachary Swartz, and Gary Wessel
13. Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata
Samantha Cihal and David Carroll
14. Perturbations to the Hedgehog Pathway in Sea Urchin Embryos
Jacob F. Warner and David R. McClay
15. Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo
Jolanta Kisielewska and Michael Whitaker
16. Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte
Maturation in Marine Nemertean Worms
Jose R. Escalona and Stephen A. Stricker
17. Multi-color Labeling in Developmental Gene Regulatory Network Analysis
Aditya J. Sethi, Robert C. Angerer and Lynne M. Angerer
18. A 96-well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay
Julio B. J. Harvey
19. Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines
Michelle M. Roux and Kathy R. Foltz
20. Tools for Sea Urchin Genomic Analysis
R. Andrew Cameron
21. Antibody Inhibition of Protein Activity in Starfish Oocytes
Eiichi Okumura, Masatoshi Hara and Takeo Kishimoto
22. Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish
Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, and Tetsuo Toraya
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