Patch-clamp methods and protocols

著者

• Martina, Marzia
• Taverna, Stefano

書誌事項

Patch-clamp methods and protocols

edited by Marzia Martina, Stefano Taverna

（Methods in molecular biology / John M. Walker, series editor, 1183）（Springer protocols）

Humana Press, c2014

2nd edition

• : [hardback]

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Patch-Cla mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

目次

1. History of Electrophysiology and the Patch-Clamp Alexej Verkhratysky and Vladimir Parpura Part I. Pharmacology 2. Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications Nate Glasgow and Jon W. Johnson 3. Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers Chris G. Parsons and Kate E. Gilling 4. State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening Sonja Stoelzle-Feix 5. Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch (R) 96 Using Instant Frozen Cells Liudmila Polonchuk 6. Planar Patch Clamp for Neuronal Networks-Considerations and Future Perspectives Alessandro Bosca, Marzia Martina, and Christophe Py Part II. Physiology 7. Methods for the Study of Synaptic Receptor Functional Properties Enrica Maria Petrini and Andrea Barberis 8. Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity Maria Toledo-Rodriguez and Henry Markram 9. Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique Romain Lefebvre, Sandrine Pouvreau, Claude Collet, B runo Allard, and Vincent Jacquemond 10. Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability Joshua L. Plotkin and James D. Surmeier 11. Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices Giovanni Russo and Stefano Taverna 12. Outside-Out 'Sniffer-patch' Clamp Technique for in situ Measures of Neurotransmitter Release Emilie Muller-Chretien 13. A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices Cary Soares, Kevin F.H. Lee, Denise Cook, and Jean-Claude Beique 14. Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics Jonathan T. Ting, Tanya L. Daigle, Qian Chen, and Guoping Feng 15. Synaptic Connectivity in Engineered Neuronal Networks Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, and James J. Hickman 16. Modeling of Action Potential Generation in NG108-15 Cells Peter Molnar and James J. Hickman 17. Whole-Cell Patch-Clamp Recordings in Freely Moving Animals Albert K. Lee, Jerome Epsztein, and Michael Brecht Part III. Biophysics 18. Pressure-Polished Borosilicate Pipettes are "Universal Sealer" Yielding Low Access Resistance and Efficient Intracellular Perfusion Marco Aquila, Mascia B enedusi, Anna Fasoli, and Giorgio Rispoli 19. Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes Romain Guinamard, Thomas Hof, and Laurent Salle II 20. Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents Geza Berecki, Arie O. Verkerk, Antoni C.G. van Ginneken, and Ronald Wilders 21. Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White, and Alan D. Dorval 22. Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes Thierry Cens and Pierre Charnet 23. Principles of Single-Channel Kinetic Analysis Feng Qin

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詳細情報

• NII書誌ID(NCID): BB16458803
• ISBN:
  • 9781493910953
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 403 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.437
• 件名:
  • LCSH : Patch-clamp techniques (Electrophysiology)
• 親書誌ID:
  • BA10794673
  • BA88873632