Monoclonal antibodies : methods and protocols

著者

• Ossipow, Vincent
• Fischer, Gustave Nicolas

書誌事項

Monoclonal antibodies : methods and protocols

edited by Vincent Ossipow, Nicolas Fischer

（Methods in molecular biology / John M. Walker, series editor, 1131）

Humana, c2014

2nd ed.

注記

ISBN for eBook: 9781627039925

Includes bibliographical references and index

内容説明・目次

内容説明

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

目次

Part I: Antibody Generation 1. Antigen Production for Monoclonal Antibody Generation Giovanni Magistrelli, Pauline Malinge 2. Method for the Generation of Antibodies Specific for Site- and Post-translational Modifications Hidemasa Goto and Masaki Inagaki3. Immunization, Hybridoma Generation and Selection for Monoclonal Antibody Production Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo 4. Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion Efthalia Chronopoulou, Alejandro Uribe-Benninghoff, Cindi R. Corbett, and Jody D. Berry 5. Generation of Rabbit Monoclonal Antibodies Pi-ChenYam and Katherine L. Knight 6. Screening Hybridomas for Cell Surface Antigens by High-throughput Homogenous Assay and Flow Cytometry Alejandro Uribe-Benninghoff, Teresa Cabral, Efthalia Chronopoulou, Jody D. Berry and Cindi R. Corbett 7. Screening and Subcloning of High Producer Transfectomas using Semi-solid Media and Automated Colony Picker Suba Dharshanan and Cheah Swee Hung 8. Design and Generation of Synthetic Antibody Libraries for Phage Display Gang Chen and Sachdev S. Sidhu 9. Selection and Screening using Antibody Phage Display Libraries Patrick Koenig and Germaine Fuh 10. Yeast Surface Display for Antibody Isolation: Library Construction, Library Screening, and Affinity Maturation James A. Van Deventer and Karl Dane Wittrup 11. Human B Cell Immortalization for Monoclonal Antibody Production Joyce Hui-Yuen, Siva Koganti,and Sumita Bhaduri-McIntosh 12. Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice Ulrike Haessler and Sai T. Reddy Part II: Antibody Expression and Purification 13. Cloning, Reformatting, and Small Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma Jeremy Loyau and Francois Rousseau 14. Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid Nicole Muller-Sienerth, Cecile Crosnier, Gavin J. Wright and Nicole Staudt 15. Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography Larry J. Cummings, Russell G. Frost and Mark A. Snyder 16. Rapid Purification of Monoclonal Antibodies using Magnetic Microspheres Pauline Malinge, Giovanni Magistrelli 17. Generation of Cell Lines for Monoclonal Antibody Production Krista Alvin and Jianxin Ye 18. Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins Martin Siegemund, Fabian Richter, Oliver Seifert, Felix Unverdorben and Roland E. Kontermann 19. Purification of Antibodies and Antibody Fragments using CaptureSelect Affinity Resins Pim Hermans, Hendrik Adams, Frank Detmers 20. Reformatting of scFv antibodies into the scFv-Fc format and their Downstream Purification Emil Bujak, Mattia Matasci, Dario Neri, and Sarah Wulhfard Part III: Anitbody Characterization and Modification 21. Antibody V and C Domain Sequence, Structure, and Interaction Analysis with Special Reference to IMGT (R) Eltaf Alamyar, Veronique Giudicelli, Patrice Duroux and Marie-Paule Lefranc 22. Measuring Antibody Affinities as well as the Active Concentration of Antigens Present on a Cell Surface Palaniswami Rathanaswami 23. Determination of Antibody Structures Robyn L. Stanfield 24. Affinity Maturation of Monoclonal Antibodies by Multi Site-Directed Mutagenesis Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo 25. Epitope Mapping with Membrane-bound Synthetic Overlapping Peptides Terumi Midoro-Horiuti and Randall M. Goldblum 26. Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange and Peptide-Panning Techniques Combined with in silico Analysis Nicola Clementi, Nicasio Mancini, Elena Criscuolo, Francesca Cappelletti, Massimo Clementi and Roberto Burioni 27. Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen Gertrudis Rojas 28. Epitope Mapping with Random Phage Display Library Terumi Midoro-Horiuti and Randall M. Goldblum 29. Epitope Mapping of Monoclonal and Polyclonal Antibodies using Bacterial Cell Surface Display Anna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg 30. Ion Exchange High Performance Liquid Chromatography (IEX-HPLC) Marie Corbier, Delphine Schrag and Sylvain Raimondi 31. Size Exclusion High Performance Liquid Chromatography (SEC-HPLC) Delphine Schrag, Marie Corbier and Sylvain Raimondi 32. N-glycosylation Characterization by Liquid Chromatography with Mass Spectrometry Song Klapoetke 33. Fc-Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and their Functional Characterization Stefanie Derer, Christian Kellner, Thies Roesner, Katja Klausz, Pia Glorius, Thomas Valerius and Matthias Peipp Part IV: Applications of Monoclonal Antibodies 34. Labeling and use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens Christoph R. Bauer 35. Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization Stephen J. Stahl, Norman R. Watts, and Paul T. Wingfield 36. Antibody Array Generation and Use Carl A.K. Borrebaeck and Christer Wingren

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BB16923889
• ISBN:
  • 9781627039918
• LCCN: 2013958232
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiv, 575 p.
• 大きさ: 27 cm.
• 分類:
  • DC23 : 616.0793
• 件名:
  • LCSH : Monoclonal antibodies
• 親書誌ID:
  • BA10794673