Exocytosis and endocytosis

著者

    • Ivanov, Andrei I.

書誌事項

Exocytosis and endocytosis

edited by Andrei I. Ivanov

（Methods in molecular biology / John M. Walker, series editor, 1174）（Springer protocols）

Humana Press, c2014

2nd ed

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

目次

Part I. An Overview of Methods for Analyzing Vesicular Trafficking 1. Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets Andrei I. Ivanov 2. Systematic Analysis of Endocytosis by Cellular Perturbations Lena Kuhling and Mario Schelhaas Part II. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis 3. Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System Oleg Varlamov 4. Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides Guoqiang Xu, Alessia Deglincerti, Jeremy S. Paige, and Samie R. Jaffrey 5. Secretion of Circular Proteins Using Sortase Karin Strijbis and Hidde Ploegh 6. Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation Xuhang Li and Mark Donowitz 7. Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis Katrin Eichelbaum and Jeroen Krijgsveld Part III. Imaging and Ultrastructural Analysis of Vesicle Trafficking in Cultured Cells 8. Probabilistic Density Maps to Study the Spatial Organization of Endocytosis Jean-Philippe Grossier, Bruno Goud, and Kristine Schauer 9. Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors Antje Schmidt, Burkhard Wiesner, Ralf Schulein, and Anke Teichmann 10. Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells Marc Fransen 11. SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells Emily H. Stoops, Glen A. Farr, Michael Hull, and Michael J. Caplan 12. FLAsH-PALM: Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling Mickael Lelek, Francesca Di Nunzio, and Christophe Zimmer 13. Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers Anton Khmelinskii and Michael Knop 14. Synchronization of Secretory Cargo Trafficking in Populations of Cells Gaelle Boncompain and Franck Perez 15. Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins Yoshimi Haga and Tadashi Suzuki 16. A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering Toru Komatsu and Takanari Inoue 17. A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays Kazumasa Ohashi and Kensaku Mizuno 18. Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy Daniel R. Passmore, Tejeshwar Rao, and Arun Anantharam 19. Nanocones to Study Initial Steps of Endocytosis Sangmoo Jeong and Milos Galic 20. A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography Nuria Jimenez and Jan A. Post 21. VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy Matthia A. Karreman and Elly G. Van Donselaar Part IV. Analysis of Exocytosis and Endocytosis in Model Organisms 22. Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network Cecilia Rodriguez-Furlan, Glenn R. Hicks, and Lorena Norambuena 23. Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans Jachen A. Solinger, Dmitry Poteryaev, and Anne Spang 24. Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers Anupma Jha and Linton M. Traub 25. Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling Jeffrey S. Dason and Milton P. Charlton 26. Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms Sudip Mondal and Sandhya P. Koushika 27. The "In Situ" Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues Arianna Bellucci, Chiara Fiorentini, Michela Zaltieri, Cristina Missale, and PierFranco Spano 28. Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy Oleg Milberg, Muhibullah Tora, Akiko Shitara, Taishin Takuma, Andrius Masedunskas, and Roberto Weigert 29. Measurement of Dynamic F-actin Changes During Exocytosis Peter Thorn

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