Cell-penetrating peptides : methods and protocols
著者
書誌事項
Cell-penetrating peptides : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 683)(Springer protocols)
Humana Press, c2011
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"Humana Press is part of Springer Science+Business Media"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology (TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.
目次
Cell-penetrating Peptides. Methods and Protocols.
Ulo Langel, Editor
Table of Contents:
Table of Contents
Preface
Contributors
Part I. Introduction
1. Classes and prediction of cell-penetrating peptides
Maria Lindgren and UElo Langel
2. Penetratin story : an overview
Edmond Dupont, Alain Prochiantz and Alain Joliot
Part II. Methods to test mechanisms of cell-penetrating peptides
3. Testing membrane interactions of CPPs
Astrid Graslund and Lena Maler
4. Interactions of amphipathic CPPs with model membranes
Sebastien Deshayes, Karidia Konate, Gudrun Aldrian, Frederic Heitz, and Gilles Divita
5. NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems
Lena Maler and Astrid Graslund
6. Measurements of the intracellular stability of CPPs
Ivo R. Ruttekolk, Wouter P. R. Verdurmen, Yi-Da Chung, and Roland Brock
7. Tools for predicting binding and insertion of CPPs into lipid bilayers
Paulo Almeida
8. Studies of proteoglycan involvement in CPP-mediated delivery
Anders Wittrup, Si-He Zhang and Mattias Belting
9. Uptake Kinetics of cell-penetrating peptides
Anders Floren, Imre Mager, and UElo Langel
10. Thermodynamics of lipid interactions with cell-penetrating peptides
Reto Sauder, Joachim Seelig and Andre Ziegler
11. Calcium and membrane repair
Caroline Palm-Apergi, and Mattias Hallbrink
12. Mapping of protein transduction pathways with fluorescent microscopy
Helin Raagel, Pille Saalik, UElo Langel, and Margus Pooga
13. Insight into cell-entry mechanisms of CPPs by electron microscopy
Kart Padari, Annely Lorents, Eija Jokitalo and Margus Pooga
14. Toxicity methods for CPPs
Per Lundin, Samir EL Andaloussi and UElo Langel
15. Comparison of CPP uptake methods
Tina Holm, Samir EL Andaloussi, and UElo Langel
16. Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery
Peter Guterstam, Samir EL Andaloussi and UElo Langel
Part III. Functionality and cell-penetrating peptides
17. Mimicry of protein function with cell-penetrating peptides
Henrik Johansson, Samir EL Andaloussi and UElo Langel
18. Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides
Alain Prochiantz
19. Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models
Sarah L. Tressel, Georgios Koukos, Boris Tchernychev, Suzanne L. Jacques, Lidija Covic, Athan Kuliopulos
20. Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display
Maliha Zahid, and Paul D. Robbins
21. Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis
Sarah Jones and John Howl
Part IV. Applications of CPPs in gene modulation
22. Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism
Rachida Abes, Andrey A. Arzumanov, Amer F. Saleh, Fatouma Said Hassane, Michael J. Gait and Bernard Lebleu
23. CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular
dystrophy
HaiFang Yin, Hong Moulton, Corinne Betts and Matthew Wood
24. PTD-DRBD siRNA delivery
Caroline Palm-Apergi, Akiko Eguchi, and Steven F. Dowdy
25. A non-covalent peptide-based strategy for siRNA delivery
Laurence Crombez, and Gilles Divita
26. Application of PepFect peptides for the delivery of splice correcting oligonucleotides
Samir EL Andaloussi, Taavi Lehto, Per Lundin, and UElo Langel
27. Internalization of nucleoside phosphates into live cells by complex formation with
different CPPs and JBS-Nucleoducin
Franziska Mussbach, Regina Pietrucha, Buerk Schaefer and Siegmund Reissmann
28. Enhanced cellular delivery of cell-penetrating peptide -peptide nucleic acid conjugates by photochemical internalization (PCI).
Takehiko Shiraishi, and Peter E. Nielsen
Part V. CPP in vivo and as future drugs
29. Identification of homing peptides using the in vivo phage display technology
Antti Rivinoja and Pirjo Laakkonen
30. Measuring the action of CPP-siRNA conjugates in the lung
Sterghios A. Moschos, Karen G. Spink, and Mark A. Lindsay
31. Intracellular delivery of nanoparticles with CPPs
Rupa Sawant, Vladimir Torchilin
32. Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery
Christian Dohmen, Ernst Wagner
33. Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application
Jose Gomez and Shigemi Matsuyama
34. PAIR technology: exon-specific RNA binding protein isolation in live cells
Thomas J. Bell, Emelia Eiriksdottir, UElo Langel and James Eberwine
35. Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates
Jonathan B. Rothbard and Lisa R. Jones
36. Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug
Ulf Tedebark, Anthony Scozzari, Oleg Werbitzky, Daniel Capaldi and Lars Holmberg
37. Application of a fusiogenic peptide GALA for intracellular delivery
Ikuhiko Nakase, Kentaro Kogure, Hideyoshi Harashima, Shiroh Futaki
38. Therapeutic Applications of Cell-Penetrating Peptides
Randolph M. Johnson, Stephen D. Harrison and Derek Maclean
39. Nonclinical and clinical experiences with CPP-based self assembling peptide systems in
topical drug development
Jacob M. Waugh, Jane Lee, Michael D. Dake and Dan Browne
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