Plant cold acclimation : methods and protocols
Author(s)
Bibliographic Information
Plant cold acclimation : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1166）（Springer protocols）
Humana Press, c2014
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"Humana Press is a brand of Springer"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.
Table of Contents
1. Measuring Freezing Tolerance: Survival and Regrowth Assays
Daniel Z. Skinner and Kimberly Garland-Campbell
2. Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays
Anja Thalhammer, Dirk K. Hincha and Ellen Zuther
3. Conducting Field Trials for Freezing Tolerance Breeding in Cereals
Luigi Cattivelli
4. A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance
Annick Bertrand, Yves Castonguay and Josee Bourassa
5. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation
Evelyne Teoule and Carine Gery
6. Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions
Andrey Malyshev, Hugh A. L. Henry and Juergen Kreyling
7. Identification of Arabidopsis Mutants with Altered Freezing Tolerance
Carlos Perea-Resa and Julio Salinas
8. Infrared Thermal Analysis of Plant Freezing Processes
Gilbert Neuner and Edith Kuprian
9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues
Seizo Fujikawa and Keita Endoh
10. Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images
David P. Livingston III and Tan D. Tuong
11. Proteomic Approaches to Identify Cold Regulated Soluble Proteins
Stefanie Doell, Rico Lippmann and Hans-Peter Mock
12. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins
Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura and Matsuo Uemura
13. Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry
Frederik Dethloff, Alexander Erban, Isabel Orf, Jessica Alpers, Ines Fehrle, Olga Beine-Golovchuk, Stefanie Schmidt, Jens Schwachtje and Joachim Kopka
14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition
Hieu Sy Vu, Sunitha Shiva, Aaron Smalter Hall and Ruth Welti
15. Quantification of Superoxide and Hydrogen Peroxide in Leaves
Ilona Juszczak and Margarete Baier
16. Estimating Ice Encasement Tolerance of Herbage Plants
Bjarni E. Gudleifsson and Brynhildur Bjarnadottir
17. Characterization of Ice Binding Proteins from Sea Ice Algae
Maddalena Bayer-Giraldi, EonSeon Jin and Peter W. Wilson
18. Isolation and Characterization of Ice Binding Proteins from Higher Plants Adam J. Middleton, Barbara Vanderbeld, Melissa Bredow, Heather Tomalty, Peter L. Davies and Virginia K. Walker
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