Immune receptors : methods and protocols
著者
書誌事項
Immune receptors : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 748）（Springer protocols）
Humana Press, c2011
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system. In Immune Receptors: Methods and Protocols, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions. These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immune Receptors: Methods and Protocols offers technical descriptions and protocols that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.
目次
1. Screening for Ligands of C-Type Lectin-Like Receptors
Elwira Pyz and Gordon D. Brown
2. Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR)
Gang Xu, Satoshi Tasumi, and Zeev Pancer
3. Identification of Scavenger Receptor Ligands
Claudine Neyen, Annette Pluddemann, and Siamon Gordon
4. Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions
John P. Cannon, Marci O'Driscoll, and Gary W. Litman
5. Innate Immune Receptors for Nucleic Acids
Andrea Stutz, Damien Bertheloot, and Eicke Latz
6. Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance
Kimiko Kuroki and Katsumi Maenaka
7. Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2
Birte Zurek, Harald Bielig, and Thomas A. Kufer
8. Determining Fc RI Diffusional Dynamics Via Single Quantum Dot Tracking
Diane S. Lidke, Shalini T. Low-Nam, Patrick J. Cutler, and Keith A. Lidke
9. Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis
Patricia Mero and James W.D. Booth
10. Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1
Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson
11. Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching
Kristen Silver and Rene E. Harrison
12. Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy
Bjoern F. Lillemeier and Mark M. Davis
13. Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis
Michal Bohdanowicz and Gregory D. Fairn
14. Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling
Alexis J. Torres, David Holowka, and Barbara A. Baird
15. CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens
Peter Chen, Aruz Mesci, and James R. Carlyle
16. Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics
Yunee Kim, Sarah Elschenbroich, Parveen Sharma, Lusia Sepiashvili, Anthony O. Gramolini, and Thomas Kislinger
17. Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants
Larry J. Dishaw, M. Gail Mueller, Robert N. Haire, and Gary W. Litman
18. Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
Nagarama Kothapalli and Sebastian D. Fugmann
19. Characterizing Immune Receptors from New Genome Sequences
Katherine M. Buckley and Jonathan P. Rast
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