Plant virology protocols : new approaches to detect viruses and host responses
著者
書誌事項
Plant virology protocols : new approaches to detect viruses and host responses
（Methods in molecular biology / John M. Walker, series editor, 1236）（Springer protocols）
Humana Press, c2015
3rd. ed
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注記
Includes index
内容説明・目次
内容説明
Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.
目次
1. Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method
Hanako Shimura, Kazuyoshi Furuta, and Chikara Masuta
2. RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers
Tyler D. B. MacKenzie, Xianzhou Nie, and Mathuresh Singh
3. New Method to Isolate Total dsRNA
Go Atsumi, Ken-Taro Sekine, and Kappei Kobayashi
4. Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses
Giorgio Gambino
5. Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)
Takahide Sasaya
6. Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector
Emanuela Noris and Laura Miozzi
7. Detection and Analysis of Non-Retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes
Hideki Kondo, Sotaro Chiba and Nobuhiro Suzuki
8. Detection of Plant Viruses in Natural Environments by Using RNA-Seq
Atsushi J. Nagano, Mie N. Honjo, Motohiro Mihara, Masanao Sato, and Hiroshi Kudoh
9. Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA
Yuan-Yuan Fang, Neil A. Smith, Jian-Hua Zhao, Joanne R. M. Lee, Hui-Shan Guo, and Ming-Bo Wang
10. Drawing siRNAs of Viral Origin out from Plant siRNAs Libraries
Laura Miozzi and Vitantonio Pantaleo
11. Viral Detection by High-Throughput Sequencing
Daisuke Motooka, Shota Nakamura, Katsuro Hagiwara, and Takaaki Nakaya
12. Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing
Charith Raj Adkar-Purushothama, Zhixiang Zhang, Shifang Li, and Teruo Sano
13. Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing
Tatsuhiro Ezawa, Yoji Ikeda, Hanako Shimura, and Chikara Masuta
14. SuperSAGE as an Analytical Tool for Host and Viral Gene Expression
Hideo Matsumura, Detlev H. Kruger, Gunter Kahl, and Ryohei Terauchi
15. Microarray Analysis of R-Gene-Mediated Resistance to Viruses
Takeaki Ishihara, Yukiyo Sato, and Hideki Takahashi
16. Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus
Kenji S. Nakahara, Kei Nishino, and Ichiro Uyeda
17. Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors
Chunjiang Li and Nobuyuki Yoshikawa
18. Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones
Tatsuya Nagata and Alice Kazuko Inoue-Nagata
19. Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control
Andreas E. Voloudakis, Maria C. Holeva, Peter L. Sarin, Dennis H. Bamford, Marisol Vargas, Minna M. Poranen, and Francisco Tenllado
20. Detection of Plant Virus in Meristem by Immunohistochemistry and In Situ Hybridization
Tomofumi Mochizuki and Satoshi T. Ohki
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