Methods in membrane lipids

著者

• Owen, Dylan M.

書誌事項

Methods in membrane lipids

edited by Dylan M. Owen

（Methods in molecular biology / John M. Walker, series editor, 1232）（Springer protocols）

Humana Press, c2015

2nd ed

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

目次

1. Introduction to Membrane Lipids Richard M. Epand 2. Introduction: Membrane properties (Good) for Life Marek Cebecauer 3. Introduction To Fluorescence Probing Of Biological Membranes Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mely 4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs) Charles Cranfield, Sonia Carne, Boris Martinac, and Bruce Cornell 5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells Deborah A. Brown 6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning KR Levental and I Levental 7. Asymmetric Giant Lipid Vesicle Fabrication Peichi C. Hu and Noah Malmstadt 8. Cholesterol Depletion using Methyl-b-cyclodextrin Saleemulla Mahammad and Ingela Parmryd 9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems G. Ekin Atilla-Gokcumen and Ulrike S. Eggert 10. Imaging Membrane Order using Environmentally Sensitive Fluorophores G Ashdown and DM Owen 11. 3D Super-resolution Imaging by Localisation Microscopy Astrid Magenau and Katharina Gaus 12. Electron Microscopy Methods for Studying Plasma Membranes Alison J. Beckett and Ian A. Prior 13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas 14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order Richard KP Benninger 15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes Eduard Hermann, Jonas Ries, and Ana J. Garcia-Saez 16. X-ray Diffraction of Lipid Model Membranes Arwen I.I. Tyler, Robert V. Law, and John M. Seddon 17. Solid State NMR of Lipid Model Membranes Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law 18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications Michael Carnell, Alex Macmillan, and Renee Whan 19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton 20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations Semen O. Yesylevskyy and Alexander P. Demchenko 21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers Svetlana Baoukina and D. Peter Tieleman

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詳細情報

• NII書誌ID(NCID): BB17410460
• ISBN:
  • 9781493917518
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 327 p.
• 大きさ: 26 cm
• 親書誌ID:
  • BA10794673
  • BA88873632