Lectins : methods and protocols

著者

• Hirabayashi, Jun

書誌事項

Lectins : methods and protocols

edited by Jun Hirabayashi

（Methods in molecular biology / John M. Walker, series editor, 1200）（Springer protocols）

Humana, c2014

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on "engineer" novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.

目次

PART I: STANDARD TECHNIQUES 1. History of Plant Lectin Research Els J.M. Van Damme 2. Fungal Lectins, A Growing Family Yuka Kobayashi, and Hirokazu Kawagishi 3. The "White Kidney Bean Incident" in Japan Haruko Ogawa, and Kimie Date 4. Hemagglutination (Inhibition) Assay Kotone Sano, and Haruko Ogawa 5. Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources Kimie Date and Haruko Ogawa 6. High-Performance Lectin Affinity Chromatography Yuka Kobayashi 7. Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography Katsuko Yamashita, and Takashi Ohkura 8. Lectin-Probed Western Blot Analysis Takeshi Sato 9. Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins Kyoko Kojima-Aikawa 10. A Simple Viral Neuraminidase-based Detection for High-throughput Screening of Viral Hemagglutinin-host Receptor Specificity Nongluk Sriwilaijaroen, and Yasuo Suzuki 11. Lectin Affinity Electrophoresis Yuka Kobayashi 12. Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans Mitsuhiro Kinoshita, and Kazuaki Kakehi 13. Basic Procedures for Lectin Flow Cytometry Kenta Moriwaki, and Eiji Miyoshi 14. Histochemical Staining Using Lectin Probes Yoshihiro Akimoto, and Hayato Kawakami 15. Equilibrium Dialysis using Chromophoric Sugar Derivatives Tomomitsu Hatakeyama 16. Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars Kanji Hori and Makoto Hirayama 17. Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions Yasuro Shinohara, and Jun-ichi Furukawa 18. Isothermal Calorimetric Analysis of Lectin-sugar Interaction Yoichi Takeda and Ichiro Matsuo 19. Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines Mamoru Mizuno PART II: EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS 20. Lectin-Based Glycomics: How and When Was the Technology Born? Jun Hirabayashi 21. Frontal Affinity Chromatography (FAC): Theory and Basic Aspects Ken-ichi Kasai 22. Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides Chihiro Sato 23. Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu 24. Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces Azusa Shibazaki, and Tohru Gonoi 25. Application of Lectin Microarray to Bacteria Including Lactobacillus casei /paracasei Strains Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi 26. Live Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito 27. Carbohydrate-binding Specificity of Lectins Using Multiplexed Glyco-bead Array Kazuo Yamamoto 28. Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins Yu-ki Matsuno, and Akihiko Kameyama PAPRT III: TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS 29. Overall Strategy for Functional Analysis of Animal Lectins Norihito Kawasaki 30. Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System Hiroaki Tateno 31. Potential Usage for in vivo Lectin Screening in Live Animals Utilized by Cell Surface Mimetic Glyco-nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs) Maho Amano, Hiroshi Hinou, Risho Miyoshi, and Shin-Ichiro Nishimura 32. Remodeling Cell Surface Glycans using Glycosylation Inhibitors Norihito Kawasaki 33. Remodeling of Glycans Using Glycosyltransferase Genes Yuko Naito-Matsui, and Hiromu Takematsu 34. Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression) Motohiro Nonaka, and Toshisuke Kawasaki 35. Analysis of L-selectin-mediated Cellular Interactions under Flow Conditions Hiroto Kawashima 36. Assessment of weak sugar-binding ability using lectin tetramer and membrane-based glycans Kazuo Yamamoto PART IV: STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS 37. Perspectives in Glycomics and Lectin Engineering Jan Tkac, Tomas Bertok, Jozef Nahalka, and Peter Gemeiner 38. Molecular Basis of Pandemic of Avian-Type Influenza Virus Nongluk Sriwilaijaroen, and Yasuo Suzuki 39. Basic Procedure of X-ray Crystallography for Analysis of Lectin-sugar Interactions Zui Fujimoto 40. A New Structure Determination Method of Lectins Using a Selenium-containing Sugar Ligand Hisayoshi Makyio, and Ryuichi Kato 41. NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy Hikaru Hemmi 42. Small Angle X-ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes Stephen D. Weeks, and Julie Bouckaert 43. Directed Evolution of Lectins by an Improved Error-prone PCR and Ribosome Display Method Dan Hu, Hiroaki Tateno, and Jun Hirabayashi 44. Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering Tomohisa Ogawa, and Tsuyoshi Shirai PART V: COMPREHENSIVE LISTS 45. Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities Yuka Kobayashi, Hiroaki Tateno, Haruko Okawa, Kazuo Yamamoto, and Jun Hirabayashi 46. Lectin Structures: Classification Based on the 3-D Structures Zui Fujimoto, Hiroaki Tateno, and Jun Hirabayashi

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詳細情報

• NII書誌ID(NCID): BB17632451
• ISBN:
  • 9781493912919
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiii, 613 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.69
• 件名:
  • LCSH : Lectins -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632