Plant cell expansion : methods and protocols

Author(s)

    • Estevez, José M.

Bibliographic Information

Plant cell expansion : methods and protocols

edited by José M. Estevez

(Methods in molecular biology / John M. Walker, series editor, 1242)(Springer protocols)

Humana Press, c2015

Available at  / 5 libraries

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"Humana Press is a brand of Springer"--T.p. verso

Includes bibliographical references and index

Description and Table of Contents

Description

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists .

Table of Contents

1. Penium margaritaceum as a Model Organism For Cell Wall Analysis Of Expanding Plant Cells Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Amanda Andreas, Jesper Harholt, Peter Ulvskov, Bodil Jorgensen, David S. Domozych, and William G.T. Willats 2. Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum N. Worden, V. Esteva Esteve, D. S. Domozych, and G. Drakakaki 3. Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes Cecilia Borassi, Juliana Perez Di Giorgio, Maria R. Scarpin, Jorge Muschietti, and Jose Estevez 4. Imaging of Calcium Dynamics in Pollen Tube Cytoplasm Maria Laura Barberini and Jorge Muschietti 5. Live Imaging of Root Hairs Silvia M. Velasquez, Jose R. Dinneny, and Jose M. Estevez 6. Improved ROS Measurement In Root Hair Cells Paola Silvina Denita Juarez, Silvina Mangano, adn Jose M. Estevez 7. A Root Hair Assay to Expedite Cell Death Research Joanna Kacprzyk and Paul F. McCabe 8. Vacuolar Staining Methods in Plant Cells David Scheuring, Maria Schoeller, Jurgen Kleine-Vehn, and Christian Loefke 9. Live Cell Imaging Of FM4-64, A Tool For Tracing The Endocytic Pathways In Arabidopsis Root Cells Adeline Rigal, Siamsa M. Doyle, and Stephanie Robert 10. Salt-Stress Regulation Of Root System Growth And Architecture In Arabidopsis Seedlings Jose R. Dinneny 11. Quantification Of Fluorescent Reporters in Plant Cells Michael Pound, Andrew P. French, and Darren M. Wells 12. Live Cell Imaging Of The Cytoskeleton And Cell Wall Enzymes In Plant Cells Arun Sampathkumar and Raymond Wightman 13. Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins Logan Bashline and Ying Gu 14. Activation Tag Screening For Cell Expansion Genes in Arabidopsis thaliana Chaowen Xiao and Charles T. Anderson 15. Bifc For Protein-Protein Interactions and Protein Topology: Discussing An Integrative Approach For An Old Technique Giovanni Stefano, Luciana Renna, and Federica Brandizzi 16. N-Glycosylation and Plant Cell Growth Christiane Veit, Ulrike Vavra, and Richard Strasser 17. Peptide Separation Methodologies for In-depth Proteomics Sajad Majeed Zargar, Rie Kurata, Randeep Rakwal, and Yoichiro Fukao 18. Structural And Mechanical Characterization Of Growing Arabidopsis Plant Cell Walls Friederike Saxe, Ingo Burgert, and Michaela Eder

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