Riboswitches as targets and tools

著者

• Burke-Aguero, Donald H.

書誌事項

Riboswitches as targets and tools

edited by Donald H. Burke-Aguero

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 550）

Academic Press, c2015

• hbk.

注記

Includes bibliographical references and indexes

内容説明・目次

内容説明

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitches as targets and tools and contains sections on such topics as constructing and optimizing artificial riboswitches, live cell imaging and intracellular sensors with artificial riboswitches, conditional control of gene expression with artificial riboswitches, using artificial riboswitches for protein evolution and pathway optimization, and anti-riboswitch drug screens.

目次

1. Design of Transcription Regulating Riboswitches Sven Findei, Manja Wachsmuth, Mario Morl, and PeterF. Stadler 2. Ligand-Dependent Exponential Amplification of Self-Replicating RNA Enzymes Charles Olea, Jr. and Gerald F. Joyce 3. Design of Modular "Plug-and-Play" Expression Platforms Derived From Natural Riboswitches for Engineering Novel Genetically Encodable RNA Regulatory Devices Jeremiah J. Trausch and Robert Batey 4. Integrating and Amplifying Signal from Riboswitch Biosensors Michael S. Goodson, Svetlana V. Harbaugh, Yaroslav G. Chushak and Nancy Kelley-Loughnane 5. Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription. Norikazu Ichihashi, Shungo Kobori, Tetsuya Yomo 6. Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system Atsushi Ogawa 7. Chemo-enzymatic Synthesis of Selectively 13C/15N-labeled RNA for NMR Structural and Dynamics Studies Luigi J. Alvarado, Andrew P. Longhini, Regan LeBlanc, Bin Chen, Christoph Kreutz and T. Kwaku Dayie 8. In Vitro Analysis of Riboswitch-Spinach Aptamer Fusions as Metabolite-Sensing Fluorescent Biosensors Colleen A. Kellenberger and Ming C. Hammond 9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases. Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk 10. Adapting CRISPR/Cas9 for Functional Genomics Screens Abba Malina , Alexandra Katigbak , Regina Cencic , Rayelle Itoua Maiga , Francis Robert, Hisashi Miura and Jerry Pelletier 11. The Icrispr Platform for Rapid Genome Editing in Human Pluripotent Stem Cells Zengrong Zhu, Federico Gonzalez and Danwei Huangfu 12. Using Riboswitches to Regulate Gene Expression and Define Gene Function in Mycobacteria Erik Van Vlack and Jessica C. Seeliger 13. Controlling Expression of Genes in the Unicellular Alga Chlamydomonas Reinhardtii With a Vitamin-Repressible Riboswitch Silvia Ramundo and Jean-David Rochaix 14. Conditional Control of Gene Expression by Synthetic Riboswitches in Streptomyces Coelicolor Martin M. Rudolph, Michael-Paul Vockenhuber und Beatrix Suess 15. Engineering of Ribozyme-Based Aminoglycoside Switches of Gene Expression by in Vivo Genetic Selection in Saccharomyces Cerevisiae Benedikt Klauser, Charlotte Rehm and Joerg S. Hartig 16. Kinetic Folding Design of Aptazyme-Regulated Expression Devices (aREDs) as Riboswitches for Metabolic Engineering David Sparkman-Yager, Rodrigo A Correa-Rojas and James M Carothers 17. Riboselector: Riboswitch-Based Synthetic Selection Device to Expedite Evolution of Metabolite-Producing Microorganisms Sungho Jang, Jina Yang, Sang Woo Seo and Gyoo Yeol Jung 18. Fluorescence Assays for Monitoring RNA-Ligand Interactions and Riboswitch-Targeted Drug Discovery Screening J. Liu, C. Zeng, S. Zhou, J.A. Means and J.V. Hines 19. Monitoring Ribosomal Frameshifting as a Platform to Screen Anti-Riboswitch Drug Candidates Chien-Hung Yu and Rene C. L. Olsthoorn

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詳細情報

• NII書誌ID(NCID): BB17862087
• ISBN:
  • 9780128011232
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam
• ページ数/冊数: xix, 423p., [24] p. of plates
• 大きさ: 24 cm
• 分類:
  • DC23 : 572.88
• 件名:
  • LCSH : Riboswitches
• 親書誌ID:
  • BA00197543