Neural tracing methods : tracing neurons and their connections
Author(s)
Bibliographic Information
Neural tracing methods : tracing neurons and their connections
（Neuromethods, 92）（Springer protocols）
Humana Press, c2015
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Note
Includes bibliographical references and index
Also published electronically
Description and Table of Contents
Description
This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.
Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.
Table of Contents
1. A Survey of Current Neuroanatomical Tracing Techniques
Floris G. Wouterlood
2. Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry
Sabrina L. Levy, Joshua J. White, and Roy V. Sillitoe
3. Retrograde Tract-Tracing 'Plus': Adding Extra Value to Retrogradely-Traced Neurons
Jose L. Lanciego
4. Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice
Brittany N. Cazakoff and Stephen D. Shea
5. Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics
Sharmin Hossain, Kaspar Podgorski, and Kurt Haas
6. Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes
Stephen D. Van Hooser, Elizabeth N. Johnson, Ye Li, Mark Mazurek, Julie H. Culp, Arani Roy, Rishabh Kasliwal, and Kelly Flavahan
7. Optogenetic Dissection of Neural Circuit Function in Behaving Animals
Carolina Gutierrez Herrera, Antoine Adamantidis, Feng Zhang, Karl Deisseroth, and Luis de Lecea
8. Remote Control of Neural Activity Using Chemical Genetics
Andrew J. Murray and Peer Wulff
9. Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits
Jonathan T. Ting and Guoping Feng
10. Engineered Rabies Virus for Transsynaptic Circuit Tracing
Jennifer Selever and Benjamin R. Arenkiel
11. Genetic Labeling of Synapses
Carlos Lois and Wolfgang Kelsch
12. Genetic Pathways to Circuit Understanding in Drosophila
Jennifer J. Esch, Yvette E. Fisher, Jonathan C.S. Leong, and Thomas R. Clandinin
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