Protein design : methods and applications
著者
書誌事項
Protein design : methods and applications
（Methods in molecular biology / John M. Walker, series editor, 1216）（Springer protocols）
Humana Press, c2014
2nd ed
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注記
Includes bibliographical references and index
Also published electronically
内容説明・目次
内容説明
Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.
目次
1. De novo Design of Stable -helices
Alexander Yakimov, Georgy Rychkov, and Michael Petukhov
2. Design of Monomeric Water-soluble b-hairpin and -sheet Peptides
M. Angeles Jimenez
3. Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-forming Peptides
Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, and Anna Mitraki
4. Posttranslational Incorporation of Non-canonical Amino Acids in the RNase S System by Semisynthetic Protein Assembly
Maika Genz and Norbert Strater
5. Design, Synthesis, and Study of Fluorinated Proteins
Benjamin C. Buer and E. Neil G. Marsh
6. High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach
Luke H. Bradley
7. Methods for Library-Scale Computational Protein Design
Lucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow
8. Symmetric Protein Architecture in Protein Design: Top-Down Symmetric Deconstruction
Liam M. Longo and Michael Blaber
9. Identification of Protein Scaffolds for Enzyme Design using Scaffold Selection
Andre C. Stiel, Kaspar Feldmeier, and Birte Hoecker
10. Computational Design of Novel Enzymes without Cofactors
Matthew D. Smith, Alexandre Zanghellini, and Daniela Grabs
11. De novo Design of Peptide Scaffolds as Novel Pre-organised Ligands for Metal-ion Coordination
Aimee J. Gamble and Anna F. A. Peacock
12. Computational Design of Metalloproteins
Avanish S. Parmar, Douglas Pike, and Vikas Nanda
13. Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein Scaffolds
Koji Oohor and Takashi Hayashi
14. Computational Redesign of Metalloenzymes for Catalyzing New Reactions
Per Jr. Greisen and Sagar D. Khare
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