Membrane proteins : engineering, purification and crystallization

著者

• Shukla, Arun K.

書誌事項

Membrane proteins : engineering, purification and crystallization

edited by Arun K. Shukla

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 557）

Academic Press, c2015

• hbk.

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

目次

Section I. Membrane Protein Engineering, Solubilization and Purification 1. Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes Simon Trowitzsch & Robert Tampe 2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs Elizabeth Mathew and Mark E Dumont 3. Membrane preparation and solubilization Ankita Roy 4. Amphipathic agents for membrane protein study Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae 5. Quantification of detergent using colorimetric methods in membrane protein crystallography Chelsy Prince , Zongchao Jia 6. Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla 7. Overexpression, Isolation, Purification and Crystallization of NhaA Etana Padan and Manish Dwivedi 8. Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coli Stefan Koester, Katharina van Pee, OEzkan Yildiz 9. Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium Symporters Satinder K. Singh and Aritra Pal Section II. Generation and Use of Antibody Fragments Against Membrane Proteins 10. Generation of recombinant antibody fragments for membrane protein crystallization Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte 11. Phage Display Selections for Affinity Reagents to Membrane Proteins in Nanodiscs Pawel K. Dominik, and Anthony A. Kossiakoff 12. Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexes Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman Section III. Biophysical Studies of Membrane Proteins 13. Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass Spectrometry Sheng Li, Su Youn Lee and Ka Young Chung 14. EPR Studies of Gating Mechanisms in Ion Channels Sudha Chakrapani 15. Magic-Angle-Spinning Solid-State NMR of Membrane Proteins Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus 16. Solution NMR Structure Determination of Polytopic a-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraints Linda Columbus and Brett Kroncke Section IV. Crystallization of Membrane Proteins 17. Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron Crystallography Yusuf M. Uddin and Ingeborg Schmidt-Krey 18. Crystallization of Membrane Proteins by Vapor Diffusion Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu 19. An empirical approach to bicelle crystallization Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham 20. Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteins Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov 21. Crystallization of Proteins from Crude Bovine Rod Outer Segments Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski 22. Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron Laser Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme Section V. Computational Approaches to Understand Membrane Proteins 23. Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity Filters Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan 24. Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the 'Mix-and-Match' Method M. Gregor Madej 25. Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing Approaches Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande

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詳細情報

• NII書誌ID(NCID): BB18634849
• ISBN:
  • 9780128021835
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam
• ページ数/冊数: xx, 624 p., [18] p. of plates
• 大きさ: 24 cm
• 分類:
  • DC23 : 572.696
• 件名:
  • LCSH : Membrane proteins
• 親書誌ID:
  • BA00197543