Far-field optical nanoscopy
Author(s)
Bibliographic Information
Far-field optical nanoscopy
（Springer series on fluorescence : methods and applications, 14）
Springer, c2015
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale.
The 2014 Chemistry Nobel Prize (R) was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Table of Contents
Part I: Optical Nanoscopy Techniques
STED Fluorescence Nanoscopy
Christian Eggeling, Stefan W. Hell
Super-Resolution Imaging Through Stochastic Switching and Localization of Single Molecules: An Overview
Ke Xu, Sang-Hee Shim, and Xiaowei Zhuang
A Practical Guide to dSTORM: Super-Resolution Imaging with Standard Fluorescent Probes
Markus Sauer
Part II: Labelling Technology for Optical Nanoscopy
Single-Molecule Photocontrol and Nanoscopy
Matthew D. Lew, Steven F. Lee, Michael A. Thompson, Hsiao-lu D. Lee, and W. E. Moerner
Probes for Nanoscopy: Fluorescent Proteins
Susan Gayda, Per Niklas Hedde, Karin Nienhaus, and G. Ulrich Nienhaus
Tailoring Fluorescent Labels for Far-Field Nanoscopy
Dmytro A. Yushchenko and Marcel P. Bruchez
Probes for Nanoscopy: Photoswitchable Fluorophores
Pedro F. Aramendia and Mariano L. Bossi
Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics, and Transient Binding
Thorben Cordes, Jan Vogelsang, Christian Steinhauer, Ingo H. Stein, Carsten Forthmann, Andreas Gietl, Jurgen J. Schmied, Guillermo P. Acuna, Sebastian Laurien, Birka Lalkens, and Philip Tinnefeld
Part III: Developments and Applications of Optical Nanoscopy
NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers
Gert De Cremer, Bert F. Sels, Dirk E. De Vos, Johan Hofkens, and Maarten B.J. Roeffaers
Counting Molecules: Toward Quantitative Imaging
Maximilian H. Ulbrich
In Vivo Tracking of Single Biomolecules: What Trajectories Tell Us About the Acting Forces
Mario Brameshuber and Gerhard J. Schutz
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