Basophils and mast cells : methods and protocols
Author(s)
Bibliographic Information
Basophils and mast cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1192）（Springer protocols）
Humana Press, c2014
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.
Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.
Table of Contents
Part I: Introduction & Historical Background
1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease
Ana Olivera and Juan Rivera
Part II: Obtaining the Cells: Purification, Culture, Counting, and Cell Lines
2. Purification of Basophils from Peripheral Human Blood
Franco H. Falcone and Bernhard F. Gibbs
3. Mast Cell Purification Protocols
Jasmine Farrington, Elizabeth P. Seward, and Peter T. Peachell
4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells
Hans Jurgen Hoffmann
5. Generation of Mast Cells from Murine Stem Cell Progenitors
Emily J. Swindle
6. Integration of the Human Dermal Mast Cell into the Organotypic Coculture Skin Model
Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc
7. The Absolute Basophil Count
Elena Borzova and Clemens A. Dahinden
8. Detection of Mast Cells and Basophils by Immunohistochemistry
Andrew F. Walls and Cornelia Amalinei
9. Mast Cell and Basophil Cell Lines: A Compendium
Egle Passante
Part III: Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters
10. Measuring Histamine and Cytokine Release from Basophils and Mast Cells
Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov
11. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques
Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo
12. Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis
Monika Bambouskova, Zuzana Hajkova, Pavel Draber, and Petr Draber
13. Use of Humanized Rat Basophil Leukaemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum
Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone
14. Gene Silencing Approaches in Mast Cells and Primary Human Basophils
Vadim V. Sumbayev and Bernhard F. Gibbs
15. Basophil Stimulation and Signaling Pathways
Edward F. Knol and Bernhard F. Gibbs
16. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells
Jose Mario Morgado, Laura Sanchez-Munoz, Cristina Teodosio, and Luis Escribano
Part IV: Whole Organism: Disease and Knockout Models
17. Identification of Murine Basophils by Flow Cytometry and Histology
Christian Schwartz and David Voehringer
18. Mast Cell Mediated Reactions In Vivo
Vladimir Andrey Gimenez-Rivera, Martin Metz, and Frank Siebenhaar
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